Comprehensive clarification of two paralogous interleukin 4/13 loci in teleost fish

Maki Ohtani, Nobuhiro Hayashi, Keiichiro Hashimoto, Teruyuki Nakanishi, J・m Dijkstra

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Interleukins 4 and 13 (IL-4 and IL-13) are related cytokines important for Th2 immune responses and encoded by adjacent genes on human chromosome 5. Efforts were made previously to detect these genes in fish, but research was hampered by a lack of sequence conservation. A Tetraodon nigrovirides (green spotted pufferfish) gene was annotated as IL-4 by Li et al. (Mol Immunol, 44:2078-2086, 2007), but this annotation was not well substantiated. However, the present study concludes that the reported pufferfish gene belongs to the IL-4/13 lineage indeed, while also describing an additional IL-4/13 copy in a paralogous genomic region. Our analyses of IL-4/13 loci in fish describe (1) genomic region history, (2) characteristic intron-exon organization, (3) deduced IL-4/13 molecules for several teleost fish species, (4) IL-4/13 lineage-specific protein motifs including a cysteine pair (pair 1), and (5) computer software predictions of a type I cytokine fold. Teleost IL-4/13 molecules have an additional cysteine pair (pair 2) or remnants thereof, which is absent in mammalian IL-4 and IL-13. We were unable to determine if the teleost IL-4/13 genes are orthologous to either IL-4 or IL-13, or if these mammalian genes separated later in evolution.

Original languageEnglish
Pages (from-to)383-397
Number of pages15
JournalImmunogenetics
Volume60
Issue number7
DOIs
Publication statusPublished - 01-07-2008

Fingerprint

Interleukin-13
Interleukin-4
Fishes
Tetraodontiformes
Genes
Cysteine
Cytokines
Chromosomes, Human, Pair 5
Amino Acid Motifs
Human Chromosomes
Introns
Exons
Software
History

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Ohtani, Maki ; Hayashi, Nobuhiro ; Hashimoto, Keiichiro ; Nakanishi, Teruyuki ; Dijkstra, J・m. / Comprehensive clarification of two paralogous interleukin 4/13 loci in teleost fish. In: Immunogenetics. 2008 ; Vol. 60, No. 7. pp. 383-397.
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Comprehensive clarification of two paralogous interleukin 4/13 loci in teleost fish. / Ohtani, Maki; Hayashi, Nobuhiro; Hashimoto, Keiichiro; Nakanishi, Teruyuki; Dijkstra, J・m.

In: Immunogenetics, Vol. 60, No. 7, 01.07.2008, p. 383-397.

Research output: Contribution to journalArticle

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