Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

Chikatoshi Kasugai, Akiko Morikawa, Yoshikazu Naiki, Naoki Koide, Takayuki Komatsu, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate-resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalImmunopharmacology and Immunotoxicology
Volume31
Issue number1
DOIs
Publication statusPublished - 03-2009

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

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