Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

Chikatoshi Kasugai; Akiko Morikawa; Yoshikazu Naiki; Naoki Koide; Takayuki Komatsu; Tomoaki Yoshida; Takashi Yokochi

doi:10.1080/08923970802438417

Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

Chikatoshi Kasugai, Akiko Morikawa, Yoshikazu Naiki, Naoki Koide, Takayuki Komatsu, Tomoaki Yoshida, Takashi Yokochi

Medical Biology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate-resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.

Original language	English
Pages (from-to)	103-107
Number of pages	5
Journal	Immunopharmacology and Immunotoxicology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1080/08923970802438417
Publication status	Published - 03-2009

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Toxicology
Pharmacology

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title = "Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells",

abstract = "The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate-resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.",

author = "Chikatoshi Kasugai and Akiko Morikawa and Yoshikazu Naiki and Naoki Koide and Takayuki Komatsu and Tomoaki Yoshida and Takashi Yokochi",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi) from the Ministry of Education, Culture, Science, Sports, Science and Technology of Japan.",

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T1 - Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

AU - Kasugai, Chikatoshi

AU - Morikawa, Akiko

AU - Naiki, Yoshikazu

AU - Koide, Naoki

AU - Komatsu, Takayuki

AU - Yoshida, Tomoaki

AU - Yokochi, Takashi

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for Scientific Research (Kakenhi) from the Ministry of Education, Culture, Science, Sports, Science and Technology of Japan.

PY - 2009/3

Y1 - 2009/3

N2 - The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate-resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.

AB - The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate-resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.

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Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

Abstract

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