Confocal endomicroscopy in patients with ulcerative colitis

Osamu Watanabe, Takafumi Ando, Osamu Maeda, Motofusa Hasegawa, Daisuke Ishikawa, Kazuhiro Ishiguro, Naoki Ohmiya, Yasumasa Niwa, Hidemi Goto

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Background: Confocal endomicroscopy is a new technology that provides microscopic images of cellular morphology in the gastrointestinal tract in vivo. In particular, confocal microendoscopy allows histological diagnosis during the endoscopic examination. We examined patients with ulcerative colitis (UC) using confocal endoscopy and investigated the features of confocal endomicroscopic images of inflamed and non-inflamed rectal mucosa. Methods: Seventeen patients with UC, 12 in the active phase and 5 in the non-active phase, and 14 non-UC control patients who had colon polyps or colon cancer underwent colonoscopy, during which fluorescence images of the rectal mucosa were obtained with confocal endomicroscopy. Histological findings of rectal biopsy specimens were compared with the microendoscopic images. Results: Confocal microendoscopy allowed observation of cellular and subcellular structures. The crypts of normal colonic mucosa were small, round and regularly arranged, and the crypt lumens of the colonic glands were small and round. The crypts of colonic mucosa in non-active UC were small, round and slightly irregular in arrangement and the crypt lumens of the colonic glands were small and round. Inflammatory cells and capillaries were visible in the lamina propria. The crypts of colonic mucosa in active UC were large, variously shaped and irregular in arrangement. The crypt lumens of the colonic glands were large and the crypt structure could not be recognized in some areas. Numerous inflammatory cells and capillaries were visible in the lamina propria. Conclusions: Images taken with the confocal microendoscope provided information that was equivalent to conventional histology, and improve our understanding of the differences in rectal tissues (crypts, capillaries and inflammatory cells) between normal controls and active and non-active UC patients during ongoing endoscopy.

Original languageEnglish
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume23
Issue numberSUPPL. 2
DOIs
Publication statusPublished - 01-01-2008
Externally publishedYes

Fingerprint

Ulcerative Colitis
Mucous Membrane
Endoscopy
Cellular Structures
Colitis
Colonoscopy
Polyps
Colonic Neoplasms
Gastrointestinal Tract
Histology
Colon
Fluorescence
Observation
Technology
Biopsy

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Watanabe, O., Ando, T., Maeda, O., Hasegawa, M., Ishikawa, D., Ishiguro, K., ... Goto, H. (2008). Confocal endomicroscopy in patients with ulcerative colitis. Journal of Gastroenterology and Hepatology (Australia), 23(SUPPL. 2). https://doi.org/10.1111/j.1440-1746.2008.05559.x
Watanabe, Osamu ; Ando, Takafumi ; Maeda, Osamu ; Hasegawa, Motofusa ; Ishikawa, Daisuke ; Ishiguro, Kazuhiro ; Ohmiya, Naoki ; Niwa, Yasumasa ; Goto, Hidemi. / Confocal endomicroscopy in patients with ulcerative colitis. In: Journal of Gastroenterology and Hepatology (Australia). 2008 ; Vol. 23, No. SUPPL. 2.
@article{5ce56eb32c974a2f8da4f752cdc13562,
title = "Confocal endomicroscopy in patients with ulcerative colitis",
abstract = "Background: Confocal endomicroscopy is a new technology that provides microscopic images of cellular morphology in the gastrointestinal tract in vivo. In particular, confocal microendoscopy allows histological diagnosis during the endoscopic examination. We examined patients with ulcerative colitis (UC) using confocal endoscopy and investigated the features of confocal endomicroscopic images of inflamed and non-inflamed rectal mucosa. Methods: Seventeen patients with UC, 12 in the active phase and 5 in the non-active phase, and 14 non-UC control patients who had colon polyps or colon cancer underwent colonoscopy, during which fluorescence images of the rectal mucosa were obtained with confocal endomicroscopy. Histological findings of rectal biopsy specimens were compared with the microendoscopic images. Results: Confocal microendoscopy allowed observation of cellular and subcellular structures. The crypts of normal colonic mucosa were small, round and regularly arranged, and the crypt lumens of the colonic glands were small and round. The crypts of colonic mucosa in non-active UC were small, round and slightly irregular in arrangement and the crypt lumens of the colonic glands were small and round. Inflammatory cells and capillaries were visible in the lamina propria. The crypts of colonic mucosa in active UC were large, variously shaped and irregular in arrangement. The crypt lumens of the colonic glands were large and the crypt structure could not be recognized in some areas. Numerous inflammatory cells and capillaries were visible in the lamina propria. Conclusions: Images taken with the confocal microendoscope provided information that was equivalent to conventional histology, and improve our understanding of the differences in rectal tissues (crypts, capillaries and inflammatory cells) between normal controls and active and non-active UC patients during ongoing endoscopy.",
author = "Osamu Watanabe and Takafumi Ando and Osamu Maeda and Motofusa Hasegawa and Daisuke Ishikawa and Kazuhiro Ishiguro and Naoki Ohmiya and Yasumasa Niwa and Hidemi Goto",
year = "2008",
month = "1",
day = "1",
doi = "10.1111/j.1440-1746.2008.05559.x",
language = "English",
volume = "23",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "SUPPL. 2",

}

Watanabe, O, Ando, T, Maeda, O, Hasegawa, M, Ishikawa, D, Ishiguro, K, Ohmiya, N, Niwa, Y & Goto, H 2008, 'Confocal endomicroscopy in patients with ulcerative colitis', Journal of Gastroenterology and Hepatology (Australia), vol. 23, no. SUPPL. 2. https://doi.org/10.1111/j.1440-1746.2008.05559.x

Confocal endomicroscopy in patients with ulcerative colitis. / Watanabe, Osamu; Ando, Takafumi; Maeda, Osamu; Hasegawa, Motofusa; Ishikawa, Daisuke; Ishiguro, Kazuhiro; Ohmiya, Naoki; Niwa, Yasumasa; Goto, Hidemi.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 23, No. SUPPL. 2, 01.01.2008.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Confocal endomicroscopy in patients with ulcerative colitis

AU - Watanabe, Osamu

AU - Ando, Takafumi

AU - Maeda, Osamu

AU - Hasegawa, Motofusa

AU - Ishikawa, Daisuke

AU - Ishiguro, Kazuhiro

AU - Ohmiya, Naoki

AU - Niwa, Yasumasa

AU - Goto, Hidemi

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Background: Confocal endomicroscopy is a new technology that provides microscopic images of cellular morphology in the gastrointestinal tract in vivo. In particular, confocal microendoscopy allows histological diagnosis during the endoscopic examination. We examined patients with ulcerative colitis (UC) using confocal endoscopy and investigated the features of confocal endomicroscopic images of inflamed and non-inflamed rectal mucosa. Methods: Seventeen patients with UC, 12 in the active phase and 5 in the non-active phase, and 14 non-UC control patients who had colon polyps or colon cancer underwent colonoscopy, during which fluorescence images of the rectal mucosa were obtained with confocal endomicroscopy. Histological findings of rectal biopsy specimens were compared with the microendoscopic images. Results: Confocal microendoscopy allowed observation of cellular and subcellular structures. The crypts of normal colonic mucosa were small, round and regularly arranged, and the crypt lumens of the colonic glands were small and round. The crypts of colonic mucosa in non-active UC were small, round and slightly irregular in arrangement and the crypt lumens of the colonic glands were small and round. Inflammatory cells and capillaries were visible in the lamina propria. The crypts of colonic mucosa in active UC were large, variously shaped and irregular in arrangement. The crypt lumens of the colonic glands were large and the crypt structure could not be recognized in some areas. Numerous inflammatory cells and capillaries were visible in the lamina propria. Conclusions: Images taken with the confocal microendoscope provided information that was equivalent to conventional histology, and improve our understanding of the differences in rectal tissues (crypts, capillaries and inflammatory cells) between normal controls and active and non-active UC patients during ongoing endoscopy.

AB - Background: Confocal endomicroscopy is a new technology that provides microscopic images of cellular morphology in the gastrointestinal tract in vivo. In particular, confocal microendoscopy allows histological diagnosis during the endoscopic examination. We examined patients with ulcerative colitis (UC) using confocal endoscopy and investigated the features of confocal endomicroscopic images of inflamed and non-inflamed rectal mucosa. Methods: Seventeen patients with UC, 12 in the active phase and 5 in the non-active phase, and 14 non-UC control patients who had colon polyps or colon cancer underwent colonoscopy, during which fluorescence images of the rectal mucosa were obtained with confocal endomicroscopy. Histological findings of rectal biopsy specimens were compared with the microendoscopic images. Results: Confocal microendoscopy allowed observation of cellular and subcellular structures. The crypts of normal colonic mucosa were small, round and regularly arranged, and the crypt lumens of the colonic glands were small and round. The crypts of colonic mucosa in non-active UC were small, round and slightly irregular in arrangement and the crypt lumens of the colonic glands were small and round. Inflammatory cells and capillaries were visible in the lamina propria. The crypts of colonic mucosa in active UC were large, variously shaped and irregular in arrangement. The crypt lumens of the colonic glands were large and the crypt structure could not be recognized in some areas. Numerous inflammatory cells and capillaries were visible in the lamina propria. Conclusions: Images taken with the confocal microendoscope provided information that was equivalent to conventional histology, and improve our understanding of the differences in rectal tissues (crypts, capillaries and inflammatory cells) between normal controls and active and non-active UC patients during ongoing endoscopy.

UR - http://www.scopus.com/inward/record.url?scp=57449083991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57449083991&partnerID=8YFLogxK

U2 - 10.1111/j.1440-1746.2008.05559.x

DO - 10.1111/j.1440-1746.2008.05559.x

M3 - Article

C2 - 19120913

AN - SCOPUS:57449083991

VL - 23

JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

IS - SUPPL. 2

ER -