Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Tetsu Mukai; Satoshi Komoto; Takeshi Kurosu; J. Alejandro Palacios; L. I. Yong-Gang; Wattana Auwanit; Masashi Tatsumi; Kazuyoshi Ikuta

doi:10.1089/088922202753747932

Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Tetsu Mukai, Satoshi Komoto, Takeshi Kurosu, J. Alejandro Palacios, L. I. Yong-Gang, Wattana Auwanit, Masashi Tatsumi, Kazuyoshi Ikuta

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

Original language	English
Pages (from-to)	585-589
Number of pages	5
Journal	AIDS Research and Human Retroviruses
Volume	18
Issue number	8
DOIs	https://doi.org/10.1089/088922202753747932
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/088922202753747932

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title = "Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1",

abstract = "An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.",

author = "Tetsu Mukai and Satoshi Komoto and Takeshi Kurosu and Palacios, {J. Alejandro} and Yong-Gang, {L. I.} and Wattana Auwanit and Masashi Tatsumi and Kazuyoshi Ikuta",

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doi = "10.1089/088922202753747932",

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journal = "AIDS Research and Human Retroviruses",

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T1 - Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

AU - Mukai, Tetsu

AU - Komoto, Satoshi

AU - Kurosu, Takeshi

AU - Palacios, J. Alejandro

AU - Yong-Gang, L. I.

AU - Auwanit, Wattana

AU - Tatsumi, Masashi

AU - Ikuta, Kazuyoshi

PY - 2002

Y1 - 2002

N2 - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

AB - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

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Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Abstract

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