Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Tetsu Mukai, Satoshi Komoto, Takeshi Kurosu, J. Alejandro Palacios, L. I. Yong-Gang, Wattana Auwanit, Masashi Tatsumi, Kazuyoshi Ikuta

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An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalAIDS Research and Human Retroviruses
Issue number8
Publication statusPublished - 01-01-2002


All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Infectious Diseases

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