Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

Tetsu Mukai, Satoshi Komoto, Takeshi Kurosu, J. Alejandro Palacios, L. I. Yong-Gang, Wattana Auwanit, Masashi Tatsumi, Kazuyoshi Ikuta

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalAIDS Research and Human Retroviruses
Volume18
Issue number8
DOIs
Publication statusPublished - 01-01-2002

Fingerprint

HIV-1
Clone Cells
Blood Cells
Viruses
Tropism
HEK293 Cells
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Mukai, Tetsu ; Komoto, Satoshi ; Kurosu, Takeshi ; Palacios, J. Alejandro ; Yong-Gang, L. I. ; Auwanit, Wattana ; Tatsumi, Masashi ; Ikuta, Kazuyoshi. / Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1. In: AIDS Research and Human Retroviruses. 2002 ; Vol. 18, No. 8. pp. 585-589.
@article{f04f07e3f0684c2a957fac908086ab04,
title = "Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1",
abstract = "An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.",
author = "Tetsu Mukai and Satoshi Komoto and Takeshi Kurosu and Palacios, {J. Alejandro} and Yong-Gang, {L. I.} and Wattana Auwanit and Masashi Tatsumi and Kazuyoshi Ikuta",
year = "2002",
month = "1",
day = "1",
doi = "10.1089/088922202753747932",
language = "English",
volume = "18",
pages = "585--589",
journal = "AIDS Research and Human Retroviruses",
issn = "0889-2229",
publisher = "Mary Ann Liebert Inc.",
number = "8",

}

Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1. / Mukai, Tetsu; Komoto, Satoshi; Kurosu, Takeshi; Palacios, J. Alejandro; Yong-Gang, L. I.; Auwanit, Wattana; Tatsumi, Masashi; Ikuta, Kazuyoshi.

In: AIDS Research and Human Retroviruses, Vol. 18, No. 8, 01.01.2002, p. 585-589.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Construction and characterization of an infectious molecular clone derived from the CRF01_AE primary isolate of HIV type 1

AU - Mukai, Tetsu

AU - Komoto, Satoshi

AU - Kurosu, Takeshi

AU - Palacios, J. Alejandro

AU - Yong-Gang, L. I.

AU - Auwanit, Wattana

AU - Tatsumi, Masashi

AU - Ikuta, Kazuyoshi

PY - 2002/1/1

Y1 - 2002/1/1

N2 - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

AB - An infectious molecular clone (named p95TNIH022) was constructed using long-range polymerase chain reaction products derived from a clinical isolate (95TNIH022) of HIV-1 CRF01_AE obtained from an asymptomatic Thai carrier in 1995. The virus in the supernatant from p95TNIH022-transfected 293T cells showed infectivity in peripheral blood mononuclear cells (PBMCs) as well as in MAGIC5 cells, which express CD4 and CCR5, but not in the original MAGI cells, indicating that p95TNIH022 is an infectious molecular clone with CCR5 tropism. Interestingly, p95TNIH022-derived virus induced profound cell killing in infected PBMCs, as in cells infected with the parental isolate.

UR - http://www.scopus.com/inward/record.url?scp=0036266794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036266794&partnerID=8YFLogxK

U2 - 10.1089/088922202753747932

DO - 10.1089/088922202753747932

M3 - Article

C2 - 12036488

AN - SCOPUS:0036266794

VL - 18

SP - 585

EP - 589

JO - AIDS Research and Human Retroviruses

JF - AIDS Research and Human Retroviruses

SN - 0889-2229

IS - 8

ER -