Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2

Shoichi Fujita, Eisei Shin, Tsutomu Nakamura, Hiroki Kurahashi, Yasufumi Kaneda, Kiyoji Tanaka, Takesada Mori, Shin ichiro Takai, Isamu Nishisho

Research output: Contribution to journalArticle

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Abstract

Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric region of chromosome 10 were developed. A Chinese hamster/human somatic cell hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were exposed to 40,000 rads of irradiation and then rescued by fusion with non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal fragments corresponding to at least one marker locus between FNRB (10p-11.2) and RBP3 (10q11.2). These hybrids were then used to map microdissection clones previously isolated and roughly mapped to this chromosomal region by fluorescence in situ hybridization (FISH). Two of the six microclones studied could be mapped to the proximity of the D10-S102 locus. These radiation hybrids are useful for the construction of refined genetic maps of the pericentromeric region of chromosome 10.

Original languageEnglish
Pages (from-to)361-370
Number of pages10
JournalThe Japanese Journal of Human Genetics
Volume38
Issue number4
DOIs
Publication statusPublished - 01-12-1993

Fingerprint

Radiation Hybrid Mapping
Chromosomes, Human, Pair 10
Chromosome Mapping
Cricetulus
Clone Cells
Microdissection
Hybrid Cells
Fluorescence In Situ Hybridization
Genetic Markers

All Science Journal Classification (ASJC) codes

  • Genetics(clinical)

Cite this

Fujita, Shoichi ; Shin, Eisei ; Nakamura, Tsutomu ; Kurahashi, Hiroki ; Kaneda, Yasufumi ; Tanaka, Kiyoji ; Mori, Takesada ; Takai, Shin ichiro ; Nishisho, Isamu. / Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2. In: The Japanese Journal of Human Genetics. 1993 ; Vol. 38, No. 4. pp. 361-370.
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abstract = "Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric region of chromosome 10 were developed. A Chinese hamster/human somatic cell hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were exposed to 40,000 rads of irradiation and then rescued by fusion with non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal fragments corresponding to at least one marker locus between FNRB (10p-11.2) and RBP3 (10q11.2). These hybrids were then used to map microdissection clones previously isolated and roughly mapped to this chromosomal region by fluorescence in situ hybridization (FISH). Two of the six microclones studied could be mapped to the proximity of the D10-S102 locus. These radiation hybrids are useful for the construction of refined genetic maps of the pericentromeric region of chromosome 10.",
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Construction of radiation-reduced hybrids and their use in mapping of microclones from chromosome 10p11.2-q11.2. / Fujita, Shoichi; Shin, Eisei; Nakamura, Tsutomu; Kurahashi, Hiroki; Kaneda, Yasufumi; Tanaka, Kiyoji; Mori, Takesada; Takai, Shin ichiro; Nishisho, Isamu.

In: The Japanese Journal of Human Genetics, Vol. 38, No. 4, 01.12.1993, p. 361-370.

Research output: Contribution to journalArticle

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AU - Fujita, Shoichi

AU - Shin, Eisei

AU - Nakamura, Tsutomu

AU - Kurahashi, Hiroki

AU - Kaneda, Yasufumi

AU - Tanaka, Kiyoji

AU - Mori, Takesada

AU - Takai, Shin ichiro

AU - Nishisho, Isamu

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N2 - Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric region of chromosome 10 were developed. A Chinese hamster/human somatic cell hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were exposed to 40,000 rads of irradiation and then rescued by fusion with non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal fragments corresponding to at least one marker locus between FNRB (10p-11.2) and RBP3 (10q11.2). These hybrids were then used to map microdissection clones previously isolated and roughly mapped to this chromosomal region by fluorescence in situ hybridization (FISH). Two of the six microclones studied could be mapped to the proximity of the D10-S102 locus. These radiation hybrids are useful for the construction of refined genetic maps of the pericentromeric region of chromosome 10.

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