Contraction of collagen matrices mediated by α2β1A and αvβ3 integrins

M. E. Cooke, T. Sakai, D. F. Mosher

Research output: Contribution to journalArticlepeer-review

85 Citations (Scopus)

Abstract

The β1-null fibroblastic cell line GD25 and its derivatives were studied to gain an understanding of the roles of β1 and β3 integrins in the initial (1-hour) contraction of collagen gels. Stable transfectants of GD25 cells expressing the β1A splice variant of β1 (β1A-GD25) did not express α2β1A and did not adhere to collagen. After transfection of α2 into β1A-GD25 cells, the α2β1A-GD25 transfectants contracted collagen gels in the presence of serum, whereas β1A-GD25 cells did not. The GD25 parental cells, however, also contracted collagen gels. Collagen gel contraction by GD25 cells was blocked by antibodies to αvβ3 or RGD-containing peptide, indicating that αvβ3 is the integrin responsible for mediation of contraction by GD25 cells. Collagen gel contraction by α2β1A-GD25 cells was not inhibited by antibodies to αvβ3 or RGD-containing peptide, but was inhibited by anti-α2 antibody. Flow cytometry demonstrated negligible expression of αvβ3 by β1A-GD25 and α2β1A-GD25 cells when compared to GD25 cells. Platelet derived growth factor (PDGF) and sphingosine-1-phosphate (S1P) enabled gel contraction by α2β1A-GD25 and GD25 cells, respectively, in the absence of serum. PDGF-stimulated contraction by α2β1A-GD25 cells was attenuated in the presence of inhibitors of phosphatidylinositol-3-kinase whereas such inhibitors had no effect on S1P-stimulated contraction by GD25 cells. These experiments using the β1-null GD25 cells and β1A and α2β1A transfectants demonstrate that α2β1A and αvβ3 independently mediate collagen gel contraction and are regulated by different serum factors and signaling pathways.

Original languageEnglish
Pages (from-to)2375-2383
Number of pages9
JournalJournal of cell science
Volume113
Issue number13
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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