Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K

Koichi Yamamoto, Mari T. Furukawa, Kazuhiro Fukumura, Arisa Kawamura, Tomoko Yamada, Hitoshi Suzuki, Tetsuro Hirose, Hiroshi Sakamoto, Kunio Inoue

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pre-mRNA splicing is widely repressed upon heat shock in eukaryotic cells. However, it has been shown that HSP105 pre-mRNA is alternatively spliced in response to heat stress. Using RNAi screening in HeLa cells, we found that RNA-binding proteins hnRNP K and PSF/SFPQ are necessary for the exon 12 exclusion of HSP105 during heat stress. Moreover, exon array analyses showed that a group of genes is alternatively spliced during heat stress in an hnRNP K-dependent manner, whereas hnRNP K is not necessary for the stress-induced alternative splicing of the remaining genes. Among the latter group, we found that SRp38/SRSF10 and SC35/SRSF2 are necessary for the inclusion of exon 13 of TNRC6A during heat stress. Thus, our study clearly showed that several RNA-binding proteins are involved in the splicing regulation in response to heat stress in mammalian cells.

Original languageEnglish
Pages (from-to)1006-1014
Number of pages9
JournalGenes to Cells
Volume21
Issue number9
DOIs
Publication statusPublished - 01-09-2016

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Heterogeneous-Nuclear Ribonucleoprotein K
Alternative Splicing
Hot Temperature
Heat-Shock Response
Exons
RNA-Binding Proteins
RNA Precursors
Genes
Eukaryotic Cells
RNA Interference
HeLa Cells
Shock

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Yamamoto, K., Furukawa, M. T., Fukumura, K., Kawamura, A., Yamada, T., Suzuki, H., ... Inoue, K. (2016). Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. Genes to Cells, 21(9), 1006-1014. https://doi.org/10.1111/gtc.12400
Yamamoto, Koichi ; Furukawa, Mari T. ; Fukumura, Kazuhiro ; Kawamura, Arisa ; Yamada, Tomoko ; Suzuki, Hitoshi ; Hirose, Tetsuro ; Sakamoto, Hiroshi ; Inoue, Kunio. / Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. In: Genes to Cells. 2016 ; Vol. 21, No. 9. pp. 1006-1014.
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Yamamoto, K, Furukawa, MT, Fukumura, K, Kawamura, A, Yamada, T, Suzuki, H, Hirose, T, Sakamoto, H & Inoue, K 2016, 'Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K', Genes to Cells, vol. 21, no. 9, pp. 1006-1014. https://doi.org/10.1111/gtc.12400

Control of the heat stress-induced alternative splicing of a subset of genes by hnRNP K. / Yamamoto, Koichi; Furukawa, Mari T.; Fukumura, Kazuhiro; Kawamura, Arisa; Yamada, Tomoko; Suzuki, Hitoshi; Hirose, Tetsuro; Sakamoto, Hiroshi; Inoue, Kunio.

In: Genes to Cells, Vol. 21, No. 9, 01.09.2016, p. 1006-1014.

Research output: Contribution to journalArticle

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AU - Yamamoto, Koichi

AU - Furukawa, Mari T.

AU - Fukumura, Kazuhiro

AU - Kawamura, Arisa

AU - Yamada, Tomoko

AU - Suzuki, Hitoshi

AU - Hirose, Tetsuro

AU - Sakamoto, Hiroshi

AU - Inoue, Kunio

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