Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus

Yoshiki Kawamura, Ken Sugata, Hidetaka Nakai, Yoshizo Asano, Masahiro Ohashi, Tomochika Kato, Naoko Nishimura, Takao Ozaki, Akiko Yui, Koki Taniguchi, Tetsushi Yoshikawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P<0.001). A weak but a significantly positive association (P=0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P=0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia.

Original languageEnglish
Pages (from-to)986-991
Number of pages6
JournalJournal of Medical Virology
Volume84
Issue number6
DOIs
Publication statusPublished - 01-06-2012

Fingerprint

Rotavirus
Matrix Metalloproteinases
Serum
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Gastroenteritis
Antigens
Tissue Inhibitor of Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Viral Antigens
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Cytokines

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Kawamura, Yoshiki ; Sugata, Ken ; Nakai, Hidetaka ; Asano, Yoshizo ; Ohashi, Masahiro ; Kato, Tomochika ; Nishimura, Naoko ; Ozaki, Takao ; Yui, Akiko ; Taniguchi, Koki ; Yoshikawa, Tetsushi. / Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus. In: Journal of Medical Virology. 2012 ; Vol. 84, No. 6. pp. 986-991.
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abstract = "Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P<0.001). A weak but a significantly positive association (P=0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P=0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia.",
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Kawamura, Y, Sugata, K, Nakai, H, Asano, Y, Ohashi, M, Kato, T, Nishimura, N, Ozaki, T, Yui, A, Taniguchi, K & Yoshikawa, T 2012, 'Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus', Journal of Medical Virology, vol. 84, no. 6, pp. 986-991. https://doi.org/10.1002/jmv.23296

Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus. / Kawamura, Yoshiki; Sugata, Ken; Nakai, Hidetaka; Asano, Yoshizo; Ohashi, Masahiro; Kato, Tomochika; Nishimura, Naoko; Ozaki, Takao; Yui, Akiko; Taniguchi, Koki; Yoshikawa, Tetsushi.

In: Journal of Medical Virology, Vol. 84, No. 6, 01.06.2012, p. 986-991.

Research output: Contribution to journalArticle

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T1 - Correlation between serum matrix metalloproteinase and antigenemia levels in patients infected with rotavirus

AU - Kawamura, Yoshiki

AU - Sugata, Ken

AU - Nakai, Hidetaka

AU - Asano, Yoshizo

AU - Ohashi, Masahiro

AU - Kato, Tomochika

AU - Nishimura, Naoko

AU - Ozaki, Takao

AU - Yui, Akiko

AU - Taniguchi, Koki

AU - Yoshikawa, Tetsushi

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N2 - Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P<0.001). A weak but a significantly positive association (P=0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P=0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia.

AB - Rotavirus (RV) antigenemia has been reported in patients with gastroenteritis; however, the exact mechanism remains unclear. In order to elucidate the mechanism of RV antigenemia, an association between RV antigenemia and matrix metalloproteinase (MMP) were analyzed. The object of this study was to elucidate the role of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in the pathogenesis of RV antigenemia. Forty children admitted to hospital with RV gastroenteritis were enrolled in this study. Paired serum samples were collected at the time of admission and discharge. Enzyme-linked immunosorbent assays (ELISA) were used to detect serum concentrations of viral antigens, MMP-1, -2, -9, -13, TIMP -1, and -2. Cytokines were measured using flow cytometric beads array. RV antigens were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-9 concentrations were significantly higher in serum collected at the time of admission than discharge (P<0.001). MMP-2 concentrations were significantly lower in serum collected at the time of admission than discharge (P<0.001). A weak but a significantly positive association (P=0.034) was observed between RV antigen and MMP-9 in serum collected at the time of admission, and inverse association was observed between RV antigen and MMP-2. In addition, a weak but significantly positive association (P=0.002) was observed between IL-6 and MMP-9. These data suggest that MMPs may contribute to the pathogenesis of RV antigenemia.

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