Correlation of p16INK4A overexpression with human papillomavirus infection in cervical adenocarcinomas

Mitsuya Ishikawa, Takuma Fujii, Nobuo Masumoto, Miyuki Saito, Makio Mukai, Ingo Nindl, Ruediger Ridder, Takeshi Fukuchi, Kaneyuki Kubushiro, Katsumi Tsukazaki, Shiro Nozawa

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54 Citations (Scopus)


As human papillomavirus (HPV) infection is the main risk factor for squamous cell carcinoma of the cervix and overexpression of p16INK4a occurs when retinoblastoma protein is inactivated by high-risk HPV, the authors studied the association of HPV infection and expression of p16INK4a in cervical adenocarcinomas. Specimens of cervical glandular neoplasias were immunostained with a p16INK4a-specific monoclonal antibody (clone E6H4). Approximately 80% of glandular neoplasms showed overexpression of p16INK4a. Exfoliated cells from 14 adenocarcinomas were further examined by p16INK4a-specific immunocytochemistry, and 12 cases showed overexpression of p16INK4a, suggesting that immunostaining for p16INK4a may be a useful diagnostic tool for cervical adenocarcinomas. The authors further examined HPV DNA in cervical adenocarcinomas with the polymerase chain reaction method. Overexpression of p16INK4a was positive in 94% of cases in which HPV16 or 18DNA was positive, a finding suggesting that HPV16 or 18 may play an important role in cervical adenocarcinomas. Overexpression of p16INK4a may be an indicator of pathogenic activity of high-risk HPVs.

Original languageEnglish
Pages (from-to)378-385
Number of pages8
JournalInternational Journal of Gynecological Pathology
Issue number4
Publication statusPublished - 10-2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Obstetrics and Gynaecology


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