CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer

Tomomitsu Tahara, Tomoyuki Shibata, Tomiyasu Arisawa, Masakatsu Nakamura, Hiromi Yamashita, Daisuke Yoshioka, Masaaki Okubo, Joh Yonemura, Yoshiteru Maeda, Naoko Maruyama, Toshiaki Kamano, Yoshio Kamiya, Hiroshi Fujita, Yoshihito Nakagawa, Mitsuo Nagasaka, Masami Iwata, Ichiro Hirata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

CpG island hypermethylation (CIHM) of tumor suppressor genes is one of the major events in the gastric carcinogenesis. We aimed to investigate the association between CIHM status of tumor suppressor genes and clinicopathological and morphological characteristics of gastric cancer. Patients and Methods: CIHM of p14, p16, Death-associated protein kinase (DAPK) and E-cadherin (CDH1) genes were determined by methylation-specific-polymerase chain Reaction in 146 gastric cancer tissues. CIHM-high was defined as three or more methylated CpG islands. Results: CIHM pf p14 was found in 70 (47.9%) cases, in 26 (17.8%) for p16, in 104 (71.2%) for CDH1 and in 127 (87.0%) for DAPK. CIHM-high was also found in 63 cases (43.2%). No association was found between between CIHM status and different staging, Lauren's subtypes, anatomic location, venous and lymphatic invasion, lymph node metastasis, distant metastasis, or peritoneal dissemination. However, among early gastric cancer cases, the depressed type with ulceration presented a significantly lower prevalence of CIHM of DAPK. In addition, Borrmann type IV cases presented significantly lower prevalence of CIHM-high among advanced gastric cancer. The Borrmann type IV cases also presented lower mean methylation number. Conclusion: The present results suggest that CIHM of DAPK and CIHM-high were associated with the morphological appearance of depressed type with ulceration in early gastric cancer, and Borrmann type IV advanced gastric cancer, respectively.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalAnticancer Research
Volume30
Issue number1
Publication statusPublished - 01-01-2010

Fingerprint

CpG Islands
Tumor Suppressor Genes
Methylation
Stomach Neoplasms
Death-Associated Protein Kinases
Neoplasm Metastasis
Cadherins
Stomach
Carcinogenesis
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Tahara, T., Shibata, T., Arisawa, T., Nakamura, M., Yamashita, H., Yoshioka, D., ... Hirata, I. (2010). CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer. Anticancer Research, 30(1), 239-244.
Tahara, Tomomitsu ; Shibata, Tomoyuki ; Arisawa, Tomiyasu ; Nakamura, Masakatsu ; Yamashita, Hiromi ; Yoshioka, Daisuke ; Okubo, Masaaki ; Yonemura, Joh ; Maeda, Yoshiteru ; Maruyama, Naoko ; Kamano, Toshiaki ; Kamiya, Yoshio ; Fujita, Hiroshi ; Nakagawa, Yoshihito ; Nagasaka, Mitsuo ; Iwata, Masami ; Hirata, Ichiro. / CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer. In: Anticancer Research. 2010 ; Vol. 30, No. 1. pp. 239-244.
@article{bf2286e74f1949bdba07fb7f9a91dd20,
title = "CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer",
abstract = "CpG island hypermethylation (CIHM) of tumor suppressor genes is one of the major events in the gastric carcinogenesis. We aimed to investigate the association between CIHM status of tumor suppressor genes and clinicopathological and morphological characteristics of gastric cancer. Patients and Methods: CIHM of p14, p16, Death-associated protein kinase (DAPK) and E-cadherin (CDH1) genes were determined by methylation-specific-polymerase chain Reaction in 146 gastric cancer tissues. CIHM-high was defined as three or more methylated CpG islands. Results: CIHM pf p14 was found in 70 (47.9{\%}) cases, in 26 (17.8{\%}) for p16, in 104 (71.2{\%}) for CDH1 and in 127 (87.0{\%}) for DAPK. CIHM-high was also found in 63 cases (43.2{\%}). No association was found between between CIHM status and different staging, Lauren's subtypes, anatomic location, venous and lymphatic invasion, lymph node metastasis, distant metastasis, or peritoneal dissemination. However, among early gastric cancer cases, the depressed type with ulceration presented a significantly lower prevalence of CIHM of DAPK. In addition, Borrmann type IV cases presented significantly lower prevalence of CIHM-high among advanced gastric cancer. The Borrmann type IV cases also presented lower mean methylation number. Conclusion: The present results suggest that CIHM of DAPK and CIHM-high were associated with the morphological appearance of depressed type with ulceration in early gastric cancer, and Borrmann type IV advanced gastric cancer, respectively.",
author = "Tomomitsu Tahara and Tomoyuki Shibata and Tomiyasu Arisawa and Masakatsu Nakamura and Hiromi Yamashita and Daisuke Yoshioka and Masaaki Okubo and Joh Yonemura and Yoshiteru Maeda and Naoko Maruyama and Toshiaki Kamano and Yoshio Kamiya and Hiroshi Fujita and Yoshihito Nakagawa and Mitsuo Nagasaka and Masami Iwata and Ichiro Hirata",
year = "2010",
month = "1",
day = "1",
language = "English",
volume = "30",
pages = "239--244",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "1",

}

Tahara, T, Shibata, T, Arisawa, T, Nakamura, M, Yamashita, H, Yoshioka, D, Okubo, M, Yonemura, J, Maeda, Y, Maruyama, N, Kamano, T, Kamiya, Y, Fujita, H, Nakagawa, Y, Nagasaka, M, Iwata, M & Hirata, I 2010, 'CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer', Anticancer Research, vol. 30, no. 1, pp. 239-244.

CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer. / Tahara, Tomomitsu; Shibata, Tomoyuki; Arisawa, Tomiyasu; Nakamura, Masakatsu; Yamashita, Hiromi; Yoshioka, Daisuke; Okubo, Masaaki; Yonemura, Joh; Maeda, Yoshiteru; Maruyama, Naoko; Kamano, Toshiaki; Kamiya, Yoshio; Fujita, Hiroshi; Nakagawa, Yoshihito; Nagasaka, Mitsuo; Iwata, Masami; Hirata, Ichiro.

In: Anticancer Research, Vol. 30, No. 1, 01.01.2010, p. 239-244.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CpG island promoter methylation (CIHM) status of tumor suppressor genes correlates with morphological appearances of gastric cancer

AU - Tahara, Tomomitsu

AU - Shibata, Tomoyuki

AU - Arisawa, Tomiyasu

AU - Nakamura, Masakatsu

AU - Yamashita, Hiromi

AU - Yoshioka, Daisuke

AU - Okubo, Masaaki

AU - Yonemura, Joh

AU - Maeda, Yoshiteru

AU - Maruyama, Naoko

AU - Kamano, Toshiaki

AU - Kamiya, Yoshio

AU - Fujita, Hiroshi

AU - Nakagawa, Yoshihito

AU - Nagasaka, Mitsuo

AU - Iwata, Masami

AU - Hirata, Ichiro

PY - 2010/1/1

Y1 - 2010/1/1

N2 - CpG island hypermethylation (CIHM) of tumor suppressor genes is one of the major events in the gastric carcinogenesis. We aimed to investigate the association between CIHM status of tumor suppressor genes and clinicopathological and morphological characteristics of gastric cancer. Patients and Methods: CIHM of p14, p16, Death-associated protein kinase (DAPK) and E-cadherin (CDH1) genes were determined by methylation-specific-polymerase chain Reaction in 146 gastric cancer tissues. CIHM-high was defined as three or more methylated CpG islands. Results: CIHM pf p14 was found in 70 (47.9%) cases, in 26 (17.8%) for p16, in 104 (71.2%) for CDH1 and in 127 (87.0%) for DAPK. CIHM-high was also found in 63 cases (43.2%). No association was found between between CIHM status and different staging, Lauren's subtypes, anatomic location, venous and lymphatic invasion, lymph node metastasis, distant metastasis, or peritoneal dissemination. However, among early gastric cancer cases, the depressed type with ulceration presented a significantly lower prevalence of CIHM of DAPK. In addition, Borrmann type IV cases presented significantly lower prevalence of CIHM-high among advanced gastric cancer. The Borrmann type IV cases also presented lower mean methylation number. Conclusion: The present results suggest that CIHM of DAPK and CIHM-high were associated with the morphological appearance of depressed type with ulceration in early gastric cancer, and Borrmann type IV advanced gastric cancer, respectively.

AB - CpG island hypermethylation (CIHM) of tumor suppressor genes is one of the major events in the gastric carcinogenesis. We aimed to investigate the association between CIHM status of tumor suppressor genes and clinicopathological and morphological characteristics of gastric cancer. Patients and Methods: CIHM of p14, p16, Death-associated protein kinase (DAPK) and E-cadherin (CDH1) genes were determined by methylation-specific-polymerase chain Reaction in 146 gastric cancer tissues. CIHM-high was defined as three or more methylated CpG islands. Results: CIHM pf p14 was found in 70 (47.9%) cases, in 26 (17.8%) for p16, in 104 (71.2%) for CDH1 and in 127 (87.0%) for DAPK. CIHM-high was also found in 63 cases (43.2%). No association was found between between CIHM status and different staging, Lauren's subtypes, anatomic location, venous and lymphatic invasion, lymph node metastasis, distant metastasis, or peritoneal dissemination. However, among early gastric cancer cases, the depressed type with ulceration presented a significantly lower prevalence of CIHM of DAPK. In addition, Borrmann type IV cases presented significantly lower prevalence of CIHM-high among advanced gastric cancer. The Borrmann type IV cases also presented lower mean methylation number. Conclusion: The present results suggest that CIHM of DAPK and CIHM-high were associated with the morphological appearance of depressed type with ulceration in early gastric cancer, and Borrmann type IV advanced gastric cancer, respectively.

UR - http://www.scopus.com/inward/record.url?scp=77649241211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77649241211&partnerID=8YFLogxK

M3 - Article

VL - 30

SP - 239

EP - 244

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 1

ER -