Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P 1,P 4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

Shigetarou Mori; Keigo Shibayama; Jun Ichi Wachino; Yoshichika Arakawa

doi:10.1107/S174430910905444X

Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P ¹,P ⁴-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

Shigetarou Mori, Keigo Shibayama, Jun Ichi Wachino, Yoshichika Arakawa

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

A novel diadenosine 5′,5′′′-P ¹,P ⁴-tetraphosphate (Ap4A) phosphorylase (Rv2613c) from Mycobacterium tuberculosis H37Rv has been crystallized by the sitting-drop vapour-diffusion method. The crystal belonged to space group C2, with unit-cell parameters a = 101.5, b = 63.6, c = 79.1 Å, Β = 110.9°. The diffraction of the crystals extended to 1.9 Å resolution. The asymmetric unit is expected to contain two molecules of Rv2613c, with a corresponding crystal volume per protein weight (V_M) of 2.41 Å³ Da^-1 and a solvent content of 49.1%. This is the first report of a crystal of Ap4A phosphorylase.

Original language	English
Pages (from-to)	279-281
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	3
DOIs	https://doi.org/10.1107/S174430910905444X
Publication status	Published - 2010
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1107/S174430910905444X

Cite this

@article{1513c92d18bc4b4b8d148201e0cdb585,

title = "Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P 1,P 4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv",

abstract = "A novel diadenosine 5′,5′′′-P 1,P 4-tetraphosphate (Ap4A) phosphorylase (Rv2613c) from Mycobacterium tuberculosis H37Rv has been crystallized by the sitting-drop vapour-diffusion method. The crystal belonged to space group C2, with unit-cell parameters a = 101.5, b = 63.6, c = 79.1 {\AA}, Β = 110.9°. The diffraction of the crystals extended to 1.9 {\AA} resolution. The asymmetric unit is expected to contain two molecules of Rv2613c, with a corresponding crystal volume per protein weight (VM) of 2.41 {\AA}3 Da-1 and a solvent content of 49.1%. This is the first report of a crystal of Ap4A phosphorylase.",

author = "Shigetarou Mori and Keigo Shibayama and Wachino, {Jun Ichi} and Yoshichika Arakawa",

year = "2010",

doi = "10.1107/S174430910905444X",

language = "English",

volume = "66",

pages = "279--281",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "3",

}

Mori, S, Shibayama, K, Wachino, JI & Arakawa, Y 2010, 'Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P ¹,P ⁴-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 3, pp. 279-281. https://doi.org/10.1107/S174430910905444X

Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P ¹,P ⁴-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv. / Mori, Shigetarou; Shibayama, Keigo; Wachino, Jun Ichi et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 3, 2010, p. 279-281.

Research output: Contribution to journal › Article › peer-review

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T1 - Crystallization and preliminary X-ray analysis of the diadenosine 5′,5′′′-P 1,P 4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv

AU - Mori, Shigetarou

AU - Shibayama, Keigo

AU - Wachino, Jun Ichi

AU - Arakawa, Yoshichika

PY - 2010

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AB - A novel diadenosine 5′,5′′′-P 1,P 4-tetraphosphate (Ap4A) phosphorylase (Rv2613c) from Mycobacterium tuberculosis H37Rv has been crystallized by the sitting-drop vapour-diffusion method. The crystal belonged to space group C2, with unit-cell parameters a = 101.5, b = 63.6, c = 79.1 Å, Β = 110.9°. The diffraction of the crystals extended to 1.9 Å resolution. The asymmetric unit is expected to contain two molecules of Rv2613c, with a corresponding crystal volume per protein weight (VM) of 2.41 Å3 Da-1 and a solvent content of 49.1%. This is the first report of a crystal of Ap4A phosphorylase.

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