TY - JOUR
T1 - Crystallization of an R-form lipopolysaccharide from Klebsiella pneumoniae
AU - Kato, Nobuo
AU - Ohta, Michio
AU - Arakawa, Yoshichika
AU - Naito, Setsuko
AU - Sugiyama, Tsuyoshi
AU - Ito, Hideo
AU - Kido, Nobuo
AU - Sasaki, Kyoyu
AU - Asai, Junpei
PY - 1996
Y1 - 1996
N2 - An R-form lipopolysaccharide (LPS) from Klebsiella pneumoniae strain LEN- 111 (O3-:K1-) formed crystals, whose shapes were elongated hexagonal plates, trapezoid plates, and rhomboid plates, and whose greatest dimensions were 3.1 x 0.8 μm, when it was suspended in 50 mM Tris buffer at pH 8.5 containing 5 mM MgCl2 and kept at 4 C for as long as 870 days. K. pneumoniae LEN-111 synthesized LPS molecules possessing incomplete repeating units of the O-antigenic polysaccharide portion besides the R-form LPS because of a leaky characteristic, but crystals consisted exclusively of the R-form LPS. Although the size of crystals was not large enough for X-ray analysis and limited crystallographic information was available, it was suggested that the crystals consist of hexagonal lattices with an a axis of 4.62 Å and c axis of 79.8±2.6 Å. The present results showed that R-form LPS lacking the O- antigenic polysaccharide portion tends to form crystals during long-term incubation in Tris buffer at pH 8.5 containing MgCl2 at 4 C.
AB - An R-form lipopolysaccharide (LPS) from Klebsiella pneumoniae strain LEN- 111 (O3-:K1-) formed crystals, whose shapes were elongated hexagonal plates, trapezoid plates, and rhomboid plates, and whose greatest dimensions were 3.1 x 0.8 μm, when it was suspended in 50 mM Tris buffer at pH 8.5 containing 5 mM MgCl2 and kept at 4 C for as long as 870 days. K. pneumoniae LEN-111 synthesized LPS molecules possessing incomplete repeating units of the O-antigenic polysaccharide portion besides the R-form LPS because of a leaky characteristic, but crystals consisted exclusively of the R-form LPS. Although the size of crystals was not large enough for X-ray analysis and limited crystallographic information was available, it was suggested that the crystals consist of hexagonal lattices with an a axis of 4.62 Å and c axis of 79.8±2.6 Å. The present results showed that R-form LPS lacking the O- antigenic polysaccharide portion tends to form crystals during long-term incubation in Tris buffer at pH 8.5 containing MgCl2 at 4 C.
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U2 - 10.1111/j.1348-0421.1996.tb01087.x
DO - 10.1111/j.1348-0421.1996.tb01087.x
M3 - Article
C2 - 8839426
AN - SCOPUS:0029954334
SN - 0385-5600
VL - 40
SP - 407
EP - 413
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 6
ER -