Crystallographic investigation of the inhibition mode of a VIM-2 metallo-β-lactamase from Pseudomonas aeruginosa by a mercaptocarboxylate inhibitor

Yoshihiro Yamaguchi, Wanchun Jin, Kazuyo Matsunaga, Shinnji Ikemizu, Yuriko Yamagata, Jun Ichi Wachino, Naohiro Shibata, Yoshichika Arakawa, Hiromasa Kurosaki

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86 Citations (Scopus)

Abstract

The VIM-2 metallo-β-lactamase enzyme from Pseudomonas aeruginosa catalyzes the hydrolysis of most β-lactam antibiotics including carbapenems, and there are currently no potent inhibitors of such enzymes. We found rac-2-ω-phenylpropyl-3-mercaptopropionic acid, phenylC3SH, to be a potent inhibitor of VIM-2. The structure of the VIM-2-phenylC3SH complex was determined by X-ray crystallography to 2.3 Å. The structure revealed that the thiol group of phenylC3SH bridged to the two zinc(II) ions and the phenyl group interacted with Tyr67(47) on loopl near the active site, by π-π stacking interactions. The methylene group interacted with Phe61(42) located at the bottom of loopl through CH-π interactions. Dynamic movements were observed in Arg228(185) and Asn233(190) on loop2, compared with the native structure (PDB code: 1KO3). These results suggest that the above-mentioned four residues play important roles in the binding and recognition of inhibitors or substrates and in stabilizing a loop in the VIM-2 enzyme.

Original languageEnglish
Pages (from-to)6647-6653
Number of pages7
JournalJournal of Medicinal Chemistry
Volume50
Issue number26
DOIs
Publication statusPublished - 27-12-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Drug Discovery

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