C2-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt

Naoki Koide; Tsuyoshi Sugiyami; Isamu Mori; Mya Mya Mu; Tomoaki Yoshida; Takashi Yokochi

doi:10.1179/096805103125001450

C₂-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt

Naoki Koide, Tsuyoshi Sugiyami, Isamu Mori, Mya Mya Mu, Tomoaki Yoshida, Takashi Yokochi

Medical Biology

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The effect of C₂-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C₂-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C₂-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C₂-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-κB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C₂-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.

Original language	English
Pages (from-to)	85-90
Number of pages	6
Journal	Journal of Endotoxin Research
Volume	9
Issue number	2
DOIs	https://doi.org/10.1179/096805103125001450
Publication status	Published - 2003

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Molecular Biology
Cell Biology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1179/096805103125001450

Cite this

@article{01f3ba35a6b844b68cdd96d78b484513,

title = "C2-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt",

abstract = "The effect of C2-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C2-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C2-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C2-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-κB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C2-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.",

author = "Naoki Koide and Tsuyoshi Sugiyami and Isamu Mori and Mu, {Mya Mya} and Tomoaki Yoshida and Takashi Yokochi",

year = "2003",

doi = "10.1179/096805103125001450",

language = "English",

volume = "9",

pages = "85--90",

journal = "Journal of Endotoxin Research",

issn = "0968-0519",

publisher = "SAGE Publications Ltd",

number = "2",

}

TY - JOUR

T1 - C2-ceramide inhibits LPS-induced nitric oxide production in RAW 264.7 macrophage cells through down-regulating the activation of Akt

AU - Koide, Naoki

AU - Sugiyami, Tsuyoshi

AU - Mori, Isamu

AU - Mu, Mya Mya

AU - Yoshida, Tomoaki

AU - Yokochi, Takashi

PY - 2003

Y1 - 2003

N2 - The effect of C2-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C2-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C2-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C2-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-κB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C2-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.

AB - The effect of C2-ceramide, a membrane-permeable ceramide analogue, on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was studied. The non-toxic concentration of C2-ceramide inhibited LPS-induced NO production. It was due to the attenuated expression of the inducible type of NO synthase (iNOS). C2-ceramide did not influence the phosphorylation of a series of mitogen-activated protein (MAP) kinases in response to LPS. On the other hand, C2-ceramide down-regulated the phosphorylation of Akt in LPS-stimulated RAW 264.7 cells, followed by the impairment of nuclear factor (NF)-κB activation. Moreover, the Akt dominant-negative mutant inhibited LPS-induced NO production. C2-ceramide was suggested to inhibit LPS-induced NO production through down-regulating the activation of Akt.

UR - http://www.scopus.com/inward/record.url?scp=0037613604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037613604&partnerID=8YFLogxK

U2 - 10.1179/096805103125001450

DO - 10.1179/096805103125001450

M3 - Article

C2 - 12803880

AN - SCOPUS:0037613604

SN - 0968-0519

VL - 9

SP - 85

EP - 90

JO - Journal of Endotoxin Research

JF - Journal of Endotoxin Research

IS - 2

ER -