Cultured human corneal epithelial stem/progenitor cells derived from the corneal limbus

Naoki Yamamoto, Koji Hirano, Hajime Kojima, Mariko Sumitomo, Hiromi Yamashita, Masahiko Ayaki, Koki Taniguchi, Atsuhiro Tanikawa, Masayuki Horiguchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Stem/progenitor cells of the human corneal epithelium are present in the human corneal limbus, and several corneal epithelial stem/progenitor cell markers have been reported. Recently, the neurotrophin family receptors were reported to be useful markers of corneal epithelial stem/progenitor cells. Therefore, we examined an enzymatic separation method for obtaining corneal epithelial stem/progenitor cells and measuring the change in the expression of low-affinity neurotrophin receptor p75 (p75NTR), a receptor belonging to the neurotrophin family. As a result, it was found that our separation method preserved cell viability. Furthermore, p75NTR was mainly observed in epithelial basal cells as were the corneal epithelial stem/progenitor markers p63 and integrin β1. p75NTR was also observed in the cultured cells, but its frequency decreased with passage. In conclusion, we propose that our culture method will enable the culture of corneal stem cells and that it is a useful tool for elucidating the molecular basis of the niche that is necessary for the maintenance of epithelial stem cells in the corneal limbus. Furthermore, we conclude that p75NTR is a useful cell marker for evaluating the characteristics of stem/progenitor cells in culture.

Original languageEnglish
Pages (from-to)774-780
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Animal
Issue number9
Publication statusPublished - 10-2010

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology


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