Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients

Tomoko S. Kato, Kazuo Komamura, Yusuke Sata, Hatsue Ishibashi-Ueda, Yoshihiko Ikeda, Taka Aki Matsuyama, Hideo Izawa, Mitsuhiro Yokota, Toshiaki Shishido, Takeshi Nakatani, Soichiro Kitamura, Masaki Asakura, Kazuhiko Hashimura, Masafumi Kitakaze

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to investigate the relationship between rejections and gene expression of Ca2+-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR+ (n = 9) with rejection grade of 2 or higher versus group AR-(n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR+ (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR- (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR+ and group AR-; however, they were reduced in group Rec-AR+ more than in group Rec-AR- (0.83 ± 0.07 versus 0.90 ± 0.07, P = 0.034, 0.74 ± 0.06 versus 0.84 ± 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca2+-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca2+-handling proteins in heart transplant recipients.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalInternational heart journal
Volume51
Issue number4
DOIs
Publication statusPublished - 26-08-2010

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Ryanodine Receptor Calcium Release Channel
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Messenger RNA
Biopsy
Gene Expression
Proteins
Reverse Transcription
Polymerase Chain Reaction
Transplant Recipients
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Kato, Tomoko S. ; Komamura, Kazuo ; Sata, Yusuke ; Ishibashi-Ueda, Hatsue ; Ikeda, Yoshihiko ; Matsuyama, Taka Aki ; Izawa, Hideo ; Yokota, Mitsuhiro ; Shishido, Toshiaki ; Nakatani, Takeshi ; Kitamura, Soichiro ; Asakura, Masaki ; Hashimura, Kazuhiko ; Kitakaze, Masafumi. / Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients. In: International heart journal. 2010 ; Vol. 51, No. 4. pp. 259-263.
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abstract = "The aim of the present study was to investigate the relationship between rejections and gene expression of Ca2+-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR+ (n = 9) with rejection grade of 2 or higher versus group AR-(n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR+ (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR- (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR+ and group AR-; however, they were reduced in group Rec-AR+ more than in group Rec-AR- (0.83 ± 0.07 versus 0.90 ± 0.07, P = 0.034, 0.74 ± 0.06 versus 0.84 ± 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca2+-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca2+-handling proteins in heart transplant recipients.",
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Kato, TS, Komamura, K, Sata, Y, Ishibashi-Ueda, H, Ikeda, Y, Matsuyama, TA, Izawa, H, Yokota, M, Shishido, T, Nakatani, T, Kitamura, S, Asakura, M, Hashimura, K & Kitakaze, M 2010, 'Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients', International heart journal, vol. 51, no. 4, pp. 259-263. https://doi.org/10.1536/ihj.51.259

Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients. / Kato, Tomoko S.; Komamura, Kazuo; Sata, Yusuke; Ishibashi-Ueda, Hatsue; Ikeda, Yoshihiko; Matsuyama, Taka Aki; Izawa, Hideo; Yokota, Mitsuhiro; Shishido, Toshiaki; Nakatani, Takeshi; Kitamura, Soichiro; Asakura, Masaki; Hashimura, Kazuhiko; Kitakaze, Masafumi.

In: International heart journal, Vol. 51, No. 4, 26.08.2010, p. 259-263.

Research output: Contribution to journalArticle

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T1 - Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients

AU - Kato, Tomoko S.

AU - Komamura, Kazuo

AU - Sata, Yusuke

AU - Ishibashi-Ueda, Hatsue

AU - Ikeda, Yoshihiko

AU - Matsuyama, Taka Aki

AU - Izawa, Hideo

AU - Yokota, Mitsuhiro

AU - Shishido, Toshiaki

AU - Nakatani, Takeshi

AU - Kitamura, Soichiro

AU - Asakura, Masaki

AU - Hashimura, Kazuhiko

AU - Kitakaze, Masafumi

PY - 2010/8/26

Y1 - 2010/8/26

N2 - The aim of the present study was to investigate the relationship between rejections and gene expression of Ca2+-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR+ (n = 9) with rejection grade of 2 or higher versus group AR-(n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR+ (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR- (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR+ and group AR-; however, they were reduced in group Rec-AR+ more than in group Rec-AR- (0.83 ± 0.07 versus 0.90 ± 0.07, P = 0.034, 0.74 ± 0.06 versus 0.84 ± 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca2+-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca2+-handling proteins in heart transplant recipients.

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