TY - JOUR
T1 - Cumulative episodes of rejection altered myocardial sarcoplasmic reticulum Ca2+-atpase and ryanodine receptor-2 mRNA expression in heart transplant recipients
AU - Kato, Tomoko S.
AU - Komamura, Kazuo
AU - Sata, Yusuke
AU - Ishibashi-Ueda, Hatsue
AU - Ikeda, Yoshihiko
AU - Matsuyama, Taka Aki
AU - Izawa, Hideo
AU - Yokota, Mitsuhiro
AU - Shishido, Toshiaki
AU - Nakatani, Takeshi
AU - Kitamura, Soichiro
AU - Asakura, Masaki
AU - Hashimura, Kazuhiko
AU - Kitakaze, Masafumi
PY - 2010
Y1 - 2010
N2 - The aim of the present study was to investigate the relationship between rejections and gene expression of Ca2+-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR+ (n = 9) with rejection grade of 2 or higher versus group AR-(n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR+ (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR- (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR+ and group AR-; however, they were reduced in group Rec-AR+ more than in group Rec-AR- (0.83 ± 0.07 versus 0.90 ± 0.07, P = 0.034, 0.74 ± 0.06 versus 0.84 ± 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca2+-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca2+-handling proteins in heart transplant recipients.
AB - The aim of the present study was to investigate the relationship between rejections and gene expression of Ca2+-handling proteins in heart transplant recipients. Thirty-seven heart transplant recipients underwent routine endomyocardial biopsy. Levels of sarcoplasmic reticulum Ca2+-ATPase (SERCA2) and ryanodine receptor-2 mRNAs in endomyocardial tissue were quantified by a real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) method. Rejections were diagnosed according to the conventional International Society for Heart and Lung Transplantation criteria. Patients were classified as follows; group AR+ (n = 9) with rejection grade of 2 or higher versus group AR-(n = 28) with rejection grade of 0, 1a or 1b at the time of biopsy, and group Rec-AR+ (n = 6) with a history of more than 4 episodes of treatment required rejection versus group Rec-AR- (n = 31) without history of recurrent rejection. The mRNA levels of the SERCA2/GAPDH ratio and ryanodine receptor-2/GAPDH ratio were not different between group AR+ and group AR-; however, they were reduced in group Rec-AR+ more than in group Rec-AR- (0.83 ± 0.07 versus 0.90 ± 0.07, P = 0.034, 0.74 ± 0.06 versus 0.84 ± 0.10, P = 0.027, respectively). A single episode of on-going rejection would not affect myocardial Ca2+-handling proteins; however, cumulative rejection episodes might alter the gene expression of myocardial Ca2+-handling proteins in heart transplant recipients.
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U2 - 10.1536/ihj.51.259
DO - 10.1536/ihj.51.259
M3 - Article
C2 - 20716843
AN - SCOPUS:77955780153
SN - 1349-2365
VL - 51
SP - 259
EP - 263
JO - International heart journal
JF - International heart journal
IS - 4
ER -