Cutting edge: Double-stranded DNA breaks in the IgV region gene were detected at lower frequency in affinity-maturation impeded GANP-/- mice

Yousuke Kawatani, Hideya Igarashi, Takeshi Matsui, Kazuhiko Kuwahara, Satoru Fujimura, Nobukazu Okamoto, Katsumasa Takagi, Nobuo Sakaguchi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Double-stranded DNA breaks (DSBs) at the IgV region (IgV) genes might be involved in somatic hypermutation and affinity-maturation of the B cell receptor in response to T cell-dependent Ag. By ligation-mediated PCR, we studied IgV DSBs that occurred in mature germinal center B cells in response to nitrophenyl-chicken γ-globulin in a RAG1-independent, Ag-dependent, and IgV-selective manner. We quantified their levels in GANP-deficient B cells that have impaired generation of high-affinity Ab. GANP-/- B cells showed a decreased level of DSBs with blunt ends than control B cells and, on the contrary, the ganp gene transgenic (GANPTg) B cells showed an increased level. These results suggested that the level of IgV DSBs in germinal center B cells is associated with GANP expression, which is presumably required for B cell receptor affinity maturation.

Original languageEnglish
Pages (from-to)5615-5618
Number of pages4
JournalJournal of Immunology
Volume175
Issue number9
DOIs
Publication statusPublished - 01-11-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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