CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration

Takaaki Yamada, Seiji Hasegawa, Yuichi Hasebe, Mika Kawagishi-Hotta, Masaru Arima, Yohei Iwata, Tsukane Kobayashi, Shigeki Numata, Naoki Yamamoto, Satoru Nakata, Kazumitsu Sugiura, Hirohiko Akamatsu

Research output: Contribution to journalArticle

Abstract

Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. To address this issue, we investigated the role of CXCL12, a chemokine which was previously suggested to induce migration of melanocyte lineage cells, as a niche component of McSCs. Immunohistological analysis revealed that CXCL12 was highly expressed in the bulge region of human hair follicles. CXCL12 mRNA expression level was significantly lower in white hairs plucked from human scalps than in black hairs. CXCL12 attracted the migration of early-passage normal human epidermal melanocytes (eNHEMs), an in vitro model of McSCs, which had characteristics of immature melanocyte precursors. We also found that CXCL12 suppressed their differentiation. These results suggest that CXCL12 regulates differentiation of McSCs as well as their proper localization, and maintaining McSCs by regulating CXCL12 expression level in the bulge region may be a key to preventing hair graying.

LanguageEnglish
Pages55-62
Number of pages8
JournalArchives of Dermatological Research
Volume311
Issue number1
DOIs
Publication statusPublished - 22-01-2019

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Melanocytes
Stem Cells
Hair
Hair Follicle
Hair Color
Chemokine CXCL12
Melanins
Scalp
Maintenance

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Yamada, T., Hasegawa, S., Hasebe, Y., Kawagishi-Hotta, M., Arima, M., Iwata, Y., ... Akamatsu, H. (2019). CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. Archives of Dermatological Research, 311(1), 55-62. https://doi.org/10.1007/s00403-018-1880-2
Yamada, Takaaki ; Hasegawa, Seiji ; Hasebe, Yuichi ; Kawagishi-Hotta, Mika ; Arima, Masaru ; Iwata, Yohei ; Kobayashi, Tsukane ; Numata, Shigeki ; Yamamoto, Naoki ; Nakata, Satoru ; Sugiura, Kazumitsu ; Akamatsu, Hirohiko. / CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. In: Archives of Dermatological Research. 2019 ; Vol. 311, No. 1. pp. 55-62.
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abstract = "Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. To address this issue, we investigated the role of CXCL12, a chemokine which was previously suggested to induce migration of melanocyte lineage cells, as a niche component of McSCs. Immunohistological analysis revealed that CXCL12 was highly expressed in the bulge region of human hair follicles. CXCL12 mRNA expression level was significantly lower in white hairs plucked from human scalps than in black hairs. CXCL12 attracted the migration of early-passage normal human epidermal melanocytes (eNHEMs), an in vitro model of McSCs, which had characteristics of immature melanocyte precursors. We also found that CXCL12 suppressed their differentiation. These results suggest that CXCL12 regulates differentiation of McSCs as well as their proper localization, and maintaining McSCs by regulating CXCL12 expression level in the bulge region may be a key to preventing hair graying.",
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Yamada, T, Hasegawa, S, Hasebe, Y, Kawagishi-Hotta, M, Arima, M, Iwata, Y, Kobayashi, T, Numata, S, Yamamoto, N, Nakata, S, Sugiura, K & Akamatsu, H 2019, 'CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration', Archives of Dermatological Research, vol. 311, no. 1, pp. 55-62. https://doi.org/10.1007/s00403-018-1880-2

CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. / Yamada, Takaaki; Hasegawa, Seiji; Hasebe, Yuichi; Kawagishi-Hotta, Mika; Arima, Masaru; Iwata, Yohei; Kobayashi, Tsukane; Numata, Shigeki; Yamamoto, Naoki; Nakata, Satoru; Sugiura, Kazumitsu; Akamatsu, Hirohiko.

In: Archives of Dermatological Research, Vol. 311, No. 1, 22.01.2019, p. 55-62.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration

AU - Yamada, Takaaki

AU - Hasegawa, Seiji

AU - Hasebe, Yuichi

AU - Kawagishi-Hotta, Mika

AU - Arima, Masaru

AU - Iwata, Yohei

AU - Kobayashi, Tsukane

AU - Numata, Shigeki

AU - Yamamoto, Naoki

AU - Nakata, Satoru

AU - Sugiura, Kazumitsu

AU - Akamatsu, Hirohiko

PY - 2019/1/22

Y1 - 2019/1/22

N2 - Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. To address this issue, we investigated the role of CXCL12, a chemokine which was previously suggested to induce migration of melanocyte lineage cells, as a niche component of McSCs. Immunohistological analysis revealed that CXCL12 was highly expressed in the bulge region of human hair follicles. CXCL12 mRNA expression level was significantly lower in white hairs plucked from human scalps than in black hairs. CXCL12 attracted the migration of early-passage normal human epidermal melanocytes (eNHEMs), an in vitro model of McSCs, which had characteristics of immature melanocyte precursors. We also found that CXCL12 suppressed their differentiation. These results suggest that CXCL12 regulates differentiation of McSCs as well as their proper localization, and maintaining McSCs by regulating CXCL12 expression level in the bulge region may be a key to preventing hair graying.

AB - Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. To address this issue, we investigated the role of CXCL12, a chemokine which was previously suggested to induce migration of melanocyte lineage cells, as a niche component of McSCs. Immunohistological analysis revealed that CXCL12 was highly expressed in the bulge region of human hair follicles. CXCL12 mRNA expression level was significantly lower in white hairs plucked from human scalps than in black hairs. CXCL12 attracted the migration of early-passage normal human epidermal melanocytes (eNHEMs), an in vitro model of McSCs, which had characteristics of immature melanocyte precursors. We also found that CXCL12 suppressed their differentiation. These results suggest that CXCL12 regulates differentiation of McSCs as well as their proper localization, and maintaining McSCs by regulating CXCL12 expression level in the bulge region may be a key to preventing hair graying.

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