Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase

Koichi Watashi, Naoto Ishii, Makoto Hijikata, Daisuke Inoue, Takayuki Murata, Yusuke Miyanari, Kunitada Shimotohno

Research output: Contribution to journalArticle

359 Citations (Scopus)

Abstract

Viruses depend on host-derived factors for their efficient genome replication. Here, we demonstrate that a cellular peptidyl-prolyl cis-trans isomerase (PPIase), cyclophilin B (CyPB), is critical for the efficient replication of the hepatitis C virus (HCV) genome. CyPB interacted with the HCV RNA polymerase NS5B to directly stimulate its RNA binding activity. Both the RNA interference (RNAi)-mediated reduction of endogenous CyPB expression and the induced loss of NS5B binding to CyPB decreased the levels of HCV replication. Thus, CyPB functions as a stimulatory regulator of NS5B in HCV replication machinery. This regulation mechanism for viral replication identifies CyPB as a target for antiviral therapeutic strategies.

Original languageEnglish
Pages (from-to)111-122
Number of pages12
JournalMolecular Cell
Volume19
Issue number1
DOIs
Publication statusPublished - 01-07-2005

Fingerprint

DNA-Directed RNA Polymerases
Hepacivirus
Virus Replication
Genome
Peptidylprolyl Isomerase
RNA Interference
Antiviral Agents
cyclophilin B
RNA
Viruses

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Watashi, K., Ishii, N., Hijikata, M., Inoue, D., Murata, T., Miyanari, Y., & Shimotohno, K. (2005). Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. Molecular Cell, 19(1), 111-122. https://doi.org/10.1016/j.molcel.2005.05.014
Watashi, Koichi ; Ishii, Naoto ; Hijikata, Makoto ; Inoue, Daisuke ; Murata, Takayuki ; Miyanari, Yusuke ; Shimotohno, Kunitada. / Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. In: Molecular Cell. 2005 ; Vol. 19, No. 1. pp. 111-122.
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Watashi, K, Ishii, N, Hijikata, M, Inoue, D, Murata, T, Miyanari, Y & Shimotohno, K 2005, 'Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase', Molecular Cell, vol. 19, no. 1, pp. 111-122. https://doi.org/10.1016/j.molcel.2005.05.014

Cyclophilin B is a functional regulator of hepatitis C virus RNA polymerase. / Watashi, Koichi; Ishii, Naoto; Hijikata, Makoto; Inoue, Daisuke; Murata, Takayuki; Miyanari, Yusuke; Shimotohno, Kunitada.

In: Molecular Cell, Vol. 19, No. 1, 01.07.2005, p. 111-122.

Research output: Contribution to journalArticle

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