Cytokine profiling in the sub-silicone oil fluid after vitrectomy surgeries for refractory retinal diseases

Hiroki Kaneko, Kei Takayama, Tetsu Asami, Yasuki Ito, Taichi Tsunekawa, Takeshi Iwase, Yasuhito Funahashi, Shinji Ueno, Norie Nonobe, Shunsuke Yasuda, Ayana Suzumura, Hideyuki Shimizu, Reona Kimoto, Shiang Jyi Hwang, Hiroko Terasaki

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesised that the fluid in the space between the SO and the retina, named the "sub-silicone oil fluid (SOF)", enhances these biological responses. We proposed a safe method for SOF extraction. We also analysed inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and macular hole-associated retinal detachment (MHRD). Interleukin (IL)-10, IL-12p40, IL-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PVR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no difference in SOF osmotic pressure between group of each disease. These studies indicate that disease-specific SOF is a significant reflection of disease status.

Original languageEnglish
Article number2640
JournalScientific reports
Issue number1
Publication statusPublished - 01-12-2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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