Cytoskeletal alterations in lipopolysaccharide-induced bovine vascular endothelial cell injury and its prevention by sodium arsenite

Dipshikha Chakravortty, Naoki Koide, Yutaka Kato, Tsuyoshi Sugiyama, Makoto Kawai, Masako Fukada, Tomoaki Yoshida, Takashi Yokochi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Morphological changes, especially cytoskeletal alterations, in lipopolysaccharide (LPS)-induced vascular endothelial cell injury were studied by using LPS-susceptible bovine aortic endothelial cells (BAEC). BAEC in cultures with LPS showed cell rounding, shrinking, and intercellular gap formation. In those cells, LPS caused the disorganization of actin, tubulin, and vimentin. LPS also induced a reduction in the F-actin pool and an elevation in the G-actin pool. Cytoskeletal disorganization affected transendothelial permeability across the endothelial monolayer. Pretreatment of BAEC with sodium arsenite (SA) prevented alterations in LPS-induced BAEC injury. However, posttreatment with SA had no protective effect on them. SA upregulated the expression of heat shock protein in the presence of LPS. The role of SA in prevention of LPS-induced BAEC injury is discussed.

Original languageEnglish
Pages (from-to)218-225
Number of pages8
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number2
DOIs
Publication statusPublished - 01-03-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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