TY - JOUR
T1 - D-Fructose Dehydrogenase-modified Carbon Paste Electrode Containing p-Benzoquinone as a Mediated Amperometric Fructose Sensor
AU - Ikeda, Tokuji
AU - Matsushita, Fumio
AU - Senda, Mitsugi
N1 - Funding Information:
Acknowledgments. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 01560150) from the Ministry of Education, Science and Culture of Japan.
PY - 1990
Y1 - 1990
N2 - D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.
AB - D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.
UR - http://www.scopus.com/inward/record.url?scp=85004246877&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004246877&partnerID=8YFLogxK
U2 - 10.1271/bbb1961.54.2919
DO - 10.1271/bbb1961.54.2919
M3 - Article
AN - SCOPUS:85004246877
SN - 0002-1369
VL - 54
SP - 2919
EP - 2924
JO - Agricultural and Biological Chemistry
JF - Agricultural and Biological Chemistry
IS - 11
ER -