D-Fructose Dehydrogenase-modified Carbon Paste Electrode Containing p-Benzoquinone as a Mediated Amperometric Fructose Sensor

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

Research output: Contribution to journalArticle

Abstract

D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.

Original languageEnglish
Pages (from-to)2919-2924
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number11
DOIs
Publication statusPublished - 01-01-1990

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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