D-Fructose Dehydrogenase-modified Carbon Paste Electrode Containing p-Benzoquinone as a Mediated Amperometric Fructose Sensor

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

Research output: Contribution to journalArticle

Abstract

D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.

Original languageEnglish
Pages (from-to)2919-2924
Number of pages6
JournalAgricultural and Biological Chemistry
Volume54
Issue number11
DOIs
Publication statusPublished - 01-01-1990
Externally publishedYes

Fingerprint

benzoquinones
Ointments
Fructose
electrodes
sensors (equipment)
fructose
Oxidoreductases
Electrodes
Carbon
carbon
Sensors
Ascorbate Oxidase
Dialysis membranes
ascorbate oxidase
dialysis
Fruits
Ascorbic Acid
benzoquinone
Dialysis
Fruit

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

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abstract = "D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.",
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D-Fructose Dehydrogenase-modified Carbon Paste Electrode Containing p-Benzoquinone as a Mediated Amperometric Fructose Sensor. / Ikeda, Tokuji; Matsushita, Fumio; Senda, Mitsugi.

In: Agricultural and Biological Chemistry, Vol. 54, No. 11, 01.01.1990, p. 2919-2924.

Research output: Contribution to journalArticle

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T1 - D-Fructose Dehydrogenase-modified Carbon Paste Electrode Containing p-Benzoquinone as a Mediated Amperometric Fructose Sensor

AU - Ikeda, Tokuji

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AB - D-Fructose dehydrogenase was immobilized behind a dialysis membrane on a carbon paste electrode containing p-benzoquinone. The electrode showed a current response to D-fructose due to the p-benzoquinone-mediated bioelectrocatalytic oxidation of D-fructose. The dependence of the current response on the potential applied to the electrode, pH of the solution, and temperature was studied. On this basis, measurements of D-fructose were done at 0.5 V vs. Ag/AgCl, pH 4.5 and 25°C using the fructose dehydrogenase-modified carbon paste electrode. D-Fructose in fruits was measured by this electrode method, in which the interference from L-ascorbic acid was taken into account. A method of elimination of the interference, which uses ascorbate oxidase, is also described.

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