Daple deficiency causes hearing loss in adult mice by inducing defects in cochlear stereocilia and apical microtubules

Yoshiyuki Ozono, Atsushi Tamura, Shogo Nakayama, Elisa Herawati, Yukiko Hanada, Kazuya Ohata, Maki Takagishi, Masahide Takahashi, Takao Imai, Yumi Ohta, Kazuo Oshima, Takashi Sato, Hidenori Inohara, Sachiko Tsukita

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1 Citation (Scopus)

Abstract

The V-shaped arrangement of hair bundles on cochlear hair cells is critical for auditory sensing. However, regulation of hair bundle arrangements has not been fully understood. Recently, defects in hair bundle arrangement were reported in postnatal Dishevelled-associating protein (ccdc88c, alias Daple)-deficient mice. In the present study, we found that adult Daple−/− mice exhibited hearing disturbances over a broad frequency range through auditory brainstem response testing. Consistently, distorted patterns of hair bundles were detected in almost all regions, more typically in the basal region of the cochlear duct. In adult Daple−/− mice, apical microtubules were irregularly aggregated, and the number of microtubules attached to plasma membranes was decreased. Similar phenotypes were manifested upon nocodazole treatment in a wild type cochlea culture without affecting the microtubule structure of the kinocilium. These results indicate critical role of Daple in hair bundle arrangement through the orchestration of apical microtubule distribution, and thereby in hearing, especially at high frequencies.

Original languageEnglish
Article number20224
JournalScientific reports
Volume11
Issue number1
DOIs
Publication statusPublished - 12-2021

All Science Journal Classification (ASJC) codes

  • General

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