De novo non-synonymous TBL1XR1 mutation alters Wnt signaling activity

Akira Nishi, Shusuke Numata, Atsushi Tajima, Xiaolei Zhu, Koki Ito, Atsushi Saito, Yusuke Kato, Makoto Kinoshita, Shinji Shimodera, Shinji Ono, Shinichiro Ochi, Akira Imamura, Naohiro Kurotaki, Shu Ichi Ueno, Nakao Iwata, Kiyoshi Fukui, Issei Imoto, Atsushi Kamiya, Tetsuro Ohmori

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Abstract

Here we report de novo non-synonymous single-nucleotide variants (SNVs) by conducting whole exome sequencing of 18 trios consisting of Japanese patients with sporadic schizophrenia and their parents. Among nine SNVs, we explored the functional impact of the de novo mutation in TBL1XR1 [c.30 C > G (p.Phe10Leu)], a gene previously found to be associated with autism spectrum disorder and epilepsy. Protein structural analysis revealed that Phe10Leu mutation may decrease the structural stability of the TBL1XR1 protein. We demonstrate that Phe10Leu mutation alters the interaction of TBL1XR1 with N-CoR and β-catenin, which play critical roles in regulation of Wnt-mediated transcriptional activity. Consistently, TBL1XR1-mediated activation of Wnt signaling was up-regulated by Phe10Leu mutation. These results suggest that a de novo TBL1XR1 point mutation could alter Wnt/β-catenin signaling activity. Further studies are required to clarify the involvement of TBL1XR1 mutations in neuropsychiatric conditions.

Original languageEnglish
Article number2887
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 01-12-2017

All Science Journal Classification (ASJC) codes

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    Nishi, A., Numata, S., Tajima, A., Zhu, X., Ito, K., Saito, A., Kato, Y., Kinoshita, M., Shimodera, S., Ono, S., Ochi, S., Imamura, A., Kurotaki, N., Ueno, S. I., Iwata, N., Fukui, K., Imoto, I., Kamiya, A., & Ohmori, T. (2017). De novo non-synonymous TBL1XR1 mutation alters Wnt signaling activity. Scientific reports, 7(1), [2887]. https://doi.org/10.1038/s41598-017-02792-z