Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats

Bian Jieyong, Wang Zhong, Zhang Shiming, Zhou Dai, Yoko Kato, Tetsuo Kanno, Hirotoshi Sano

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Both mild hypothermia (MH) and decompressive craniectomy (CE) have been shown to have neuroprotective effects in brain ischemia. We investigated a possible effect of MH and a combination of CE and MH (CE+MH) on the changes of infarction size, DNA fragmentation, and immunoreactivities for Bcl-2 and Bax after 24 h of permanent middle cerebral artery occlusion (MCAO) in rats. For the estimation of ischemic brain injury, we calculated the infarct size of the MCA region at 24 h after the MCAO. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining was performed for the detection of DNA fragmentation. Immunoreactivities for Bcl-2 and Bax were stained. Infarction size after permanent MCAO was significantly reduced by CE+MH treatment (P<0.01). Infarction size did not change significantly by application of MH alone (P>0.05). TUNEL staining was remarkably reduced both in MH-treated animals and in CE+MH-treated animals. Immunoreactivity for Bcl-2 was greatly induced both in MH-treated animals and in CE+MH-treated animals. Induction of immunoreactivity for Bcl-2 was obviously inhibited both in MH-treated animals and in CE+MH-treated animals. It suggests that temporary MH delays infarct evolution and ameliorates neuron apoptosis but does not significantly reduce definite infarction size. CE+MH not only ameliorates neuron apoptosis but also remarkably reduces infarction size.

Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalNeurosurgical Review
Volume29
Issue number2
DOIs
Publication statusPublished - 04-2006

All Science Journal Classification (ASJC) codes

  • Surgery
  • Clinical Neurology

Fingerprint Dive into the research topics of 'Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats'. Together they form a unique fingerprint.

Cite this