Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease

Katsuhiro Onodera, Hisatoshi Sugiura, Mitsuhiro Yamada, Akira Koarai, Naoya Fujino, Satoru Yanagisawa, Rie Tanaka, Tadahisa Numakura, Shinsaku Togo, Kei Sato, Yorihiko Kyogoku, Yuichiro Hashimoto, Tatsuma Okazaki, Tsutomu Tamada, Seiichi Kobayashi, Masaru Yanai, Motohiko Miura, Yasushi Hoshikawa, Yoshinori Okada, Satoshi SuzukiMasakazu Ichinose

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rationale Cellular senescence is observed in the lungs of patients with COPD and may contribute to the disease pathogenesis. Growth differentiation factor 11 (GDF11) belongs to the transforming growth factor ß superfamily and was recently reported to be a circulating protein that may have rejuvenating effects in mice. We aimed to investigate the amounts of GDF11 in the plasma and the lungs of patients with COPD and elucidate the possible roles of GDF11 in cellular senescence. Methods The plasma levels of GDF11 were investigated in two separate cohorts by western blotting. The localisation and expression of GDF11 in the lungs were investigated by immunohistochemistry and quantitative reverse transcription PCR, respectively. The effects of GDF11 on both cigarette smoke extract (CSE)- induced cellular senescence in vitro and on elastaseinduced cellular senescence in vivo were investigated. Results The levels of plasma GDF11 in the COPD group were decreased compared with the control groups in the two independent cohorts. The levels of plasma GDF11 were significantly positively correlated with pulmonary function data. The mRNA expression of GDF11 in mesenchymal cells from the COPD group was decreased. Chronic exposure to CSE decreased the production of GDF11. Treatment with GDF11 significantly inhibited CSE-induced cellular senescence and upregulation of inflammatory mediators, partly through Smad2/3 signalling in vitro. Daily GDF11 treatment attenuated cellular senescence and airspace enlargement in an elastase-induced mouse model of emphysema. Conclusions The decrease in GDF11 may be involved in the cellular senescence observed in COPD.

Original languageEnglish
JournalThorax
DOIs
Publication statusAccepted/In press - 28-04-2017

Fingerprint

Growth Differentiation Factors
Chronic Obstructive Pulmonary Disease
Cell Aging
Smoke
Tobacco Products
Lung
Pancreatic Elastase
Emphysema
Transforming Growth Factors

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Onodera, K., Sugiura, H., Yamada, M., Koarai, A., Fujino, N., Yanagisawa, S., ... Ichinose, M. (Accepted/In press). Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease. Thorax. https://doi.org/10.1136/thoraxjnl-2016-209352
Onodera, Katsuhiro ; Sugiura, Hisatoshi ; Yamada, Mitsuhiro ; Koarai, Akira ; Fujino, Naoya ; Yanagisawa, Satoru ; Tanaka, Rie ; Numakura, Tadahisa ; Togo, Shinsaku ; Sato, Kei ; Kyogoku, Yorihiko ; Hashimoto, Yuichiro ; Okazaki, Tatsuma ; Tamada, Tsutomu ; Kobayashi, Seiichi ; Yanai, Masaru ; Miura, Motohiko ; Hoshikawa, Yasushi ; Okada, Yoshinori ; Suzuki, Satoshi ; Ichinose, Masakazu. / Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease. In: Thorax. 2017.
@article{c12cf39e0de243c0b89fdaf82ef00637,
title = "Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease",
abstract = "Rationale Cellular senescence is observed in the lungs of patients with COPD and may contribute to the disease pathogenesis. Growth differentiation factor 11 (GDF11) belongs to the transforming growth factor {\ss} superfamily and was recently reported to be a circulating protein that may have rejuvenating effects in mice. We aimed to investigate the amounts of GDF11 in the plasma and the lungs of patients with COPD and elucidate the possible roles of GDF11 in cellular senescence. Methods The plasma levels of GDF11 were investigated in two separate cohorts by western blotting. The localisation and expression of GDF11 in the lungs were investigated by immunohistochemistry and quantitative reverse transcription PCR, respectively. The effects of GDF11 on both cigarette smoke extract (CSE)- induced cellular senescence in vitro and on elastaseinduced cellular senescence in vivo were investigated. Results The levels of plasma GDF11 in the COPD group were decreased compared with the control groups in the two independent cohorts. The levels of plasma GDF11 were significantly positively correlated with pulmonary function data. The mRNA expression of GDF11 in mesenchymal cells from the COPD group was decreased. Chronic exposure to CSE decreased the production of GDF11. Treatment with GDF11 significantly inhibited CSE-induced cellular senescence and upregulation of inflammatory mediators, partly through Smad2/3 signalling in vitro. Daily GDF11 treatment attenuated cellular senescence and airspace enlargement in an elastase-induced mouse model of emphysema. Conclusions The decrease in GDF11 may be involved in the cellular senescence observed in COPD.",
author = "Katsuhiro Onodera and Hisatoshi Sugiura and Mitsuhiro Yamada and Akira Koarai and Naoya Fujino and Satoru Yanagisawa and Rie Tanaka and Tadahisa Numakura and Shinsaku Togo and Kei Sato and Yorihiko Kyogoku and Yuichiro Hashimoto and Tatsuma Okazaki and Tsutomu Tamada and Seiichi Kobayashi and Masaru Yanai and Motohiko Miura and Yasushi Hoshikawa and Yoshinori Okada and Satoshi Suzuki and Masakazu Ichinose",
year = "2017",
month = "4",
day = "28",
doi = "10.1136/thoraxjnl-2016-209352",
language = "English",
journal = "Thorax",
issn = "0040-6376",
publisher = "BMJ Publishing Group",

}

Onodera, K, Sugiura, H, Yamada, M, Koarai, A, Fujino, N, Yanagisawa, S, Tanaka, R, Numakura, T, Togo, S, Sato, K, Kyogoku, Y, Hashimoto, Y, Okazaki, T, Tamada, T, Kobayashi, S, Yanai, M, Miura, M, Hoshikawa, Y, Okada, Y, Suzuki, S & Ichinose, M 2017, 'Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease', Thorax. https://doi.org/10.1136/thoraxjnl-2016-209352

Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease. / Onodera, Katsuhiro; Sugiura, Hisatoshi; Yamada, Mitsuhiro; Koarai, Akira; Fujino, Naoya; Yanagisawa, Satoru; Tanaka, Rie; Numakura, Tadahisa; Togo, Shinsaku; Sato, Kei; Kyogoku, Yorihiko; Hashimoto, Yuichiro; Okazaki, Tatsuma; Tamada, Tsutomu; Kobayashi, Seiichi; Yanai, Masaru; Miura, Motohiko; Hoshikawa, Yasushi; Okada, Yoshinori; Suzuki, Satoshi; Ichinose, Masakazu.

In: Thorax, 28.04.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease

AU - Onodera, Katsuhiro

AU - Sugiura, Hisatoshi

AU - Yamada, Mitsuhiro

AU - Koarai, Akira

AU - Fujino, Naoya

AU - Yanagisawa, Satoru

AU - Tanaka, Rie

AU - Numakura, Tadahisa

AU - Togo, Shinsaku

AU - Sato, Kei

AU - Kyogoku, Yorihiko

AU - Hashimoto, Yuichiro

AU - Okazaki, Tatsuma

AU - Tamada, Tsutomu

AU - Kobayashi, Seiichi

AU - Yanai, Masaru

AU - Miura, Motohiko

AU - Hoshikawa, Yasushi

AU - Okada, Yoshinori

AU - Suzuki, Satoshi

AU - Ichinose, Masakazu

PY - 2017/4/28

Y1 - 2017/4/28

N2 - Rationale Cellular senescence is observed in the lungs of patients with COPD and may contribute to the disease pathogenesis. Growth differentiation factor 11 (GDF11) belongs to the transforming growth factor ß superfamily and was recently reported to be a circulating protein that may have rejuvenating effects in mice. We aimed to investigate the amounts of GDF11 in the plasma and the lungs of patients with COPD and elucidate the possible roles of GDF11 in cellular senescence. Methods The plasma levels of GDF11 were investigated in two separate cohorts by western blotting. The localisation and expression of GDF11 in the lungs were investigated by immunohistochemistry and quantitative reverse transcription PCR, respectively. The effects of GDF11 on both cigarette smoke extract (CSE)- induced cellular senescence in vitro and on elastaseinduced cellular senescence in vivo were investigated. Results The levels of plasma GDF11 in the COPD group were decreased compared with the control groups in the two independent cohorts. The levels of plasma GDF11 were significantly positively correlated with pulmonary function data. The mRNA expression of GDF11 in mesenchymal cells from the COPD group was decreased. Chronic exposure to CSE decreased the production of GDF11. Treatment with GDF11 significantly inhibited CSE-induced cellular senescence and upregulation of inflammatory mediators, partly through Smad2/3 signalling in vitro. Daily GDF11 treatment attenuated cellular senescence and airspace enlargement in an elastase-induced mouse model of emphysema. Conclusions The decrease in GDF11 may be involved in the cellular senescence observed in COPD.

AB - Rationale Cellular senescence is observed in the lungs of patients with COPD and may contribute to the disease pathogenesis. Growth differentiation factor 11 (GDF11) belongs to the transforming growth factor ß superfamily and was recently reported to be a circulating protein that may have rejuvenating effects in mice. We aimed to investigate the amounts of GDF11 in the plasma and the lungs of patients with COPD and elucidate the possible roles of GDF11 in cellular senescence. Methods The plasma levels of GDF11 were investigated in two separate cohorts by western blotting. The localisation and expression of GDF11 in the lungs were investigated by immunohistochemistry and quantitative reverse transcription PCR, respectively. The effects of GDF11 on both cigarette smoke extract (CSE)- induced cellular senescence in vitro and on elastaseinduced cellular senescence in vivo were investigated. Results The levels of plasma GDF11 in the COPD group were decreased compared with the control groups in the two independent cohorts. The levels of plasma GDF11 were significantly positively correlated with pulmonary function data. The mRNA expression of GDF11 in mesenchymal cells from the COPD group was decreased. Chronic exposure to CSE decreased the production of GDF11. Treatment with GDF11 significantly inhibited CSE-induced cellular senescence and upregulation of inflammatory mediators, partly through Smad2/3 signalling in vitro. Daily GDF11 treatment attenuated cellular senescence and airspace enlargement in an elastase-induced mouse model of emphysema. Conclusions The decrease in GDF11 may be involved in the cellular senescence observed in COPD.

UR - http://www.scopus.com/inward/record.url?scp=85028763903&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028763903&partnerID=8YFLogxK

U2 - 10.1136/thoraxjnl-2016-209352

DO - 10.1136/thoraxjnl-2016-209352

M3 - Article

C2 - 28455454

AN - SCOPUS:85028763903

JO - Thorax

JF - Thorax

SN - 0040-6376

ER -