Decreased expression of apelin in placentas from severe pre-eclampsia patients

Hiromi Inuzuka, Haruki Nishizawa, Ayaka Inagaki, Machiko Suzuki, Sayuri Ota, Hironori Miyamura, Jun Miyazaki, Takao Sekiya, Hiroki Kurahashi, Yasuhiro Udagawa

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19 Citations (Scopus)

Abstract

Objective: It is well documented that anti-angiogenic factors are likely to play essential roles in the etiology of pre-eclampsia. Apelin is a small peptide that may potentially act as an angiogenic factor. The expression of apelin was examined at the RNA and protein levels in this study. Methods: We compared the expression of apelin, examined using quantitative reverse-transcription polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay and immunostaining, between pre-eclamptic patients and normotensive controls. Results: Apelin messenger RNA is significantly decreased in pre-eclamptic placentas compared with normotensive pregnancies (p < 0.05). Apelin protein levels are also lower in pre-eclamptic placentas than the controls but higher in the maternal circulation in pre-eclampsia patients. Immunohistochemical signals for apelin and its receptor APJ were detected mainly in the cytoplasm of syncytiotrophoblasts in chorionic villi and trophoblast-lineage cells in the decidua of term placentas. In early gestation, stronger APJ signals were observed at the cellular membrane. Conclusions: A functional role of the apelin - APJ system is likely in early gestation, and this raises the possibility that a dysfunctional apelin - APJ system contributes to the onset of pre-eclampsia via decreased angiogenic activity in placental implantation.

Original languageEnglish
Pages (from-to)410-421
Number of pages12
JournalHypertension in Pregnancy
Volume32
Issue number4
DOIs
Publication statusPublished - 01-11-2013

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Obstetrics and Gynaecology

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    Inuzuka, H., Nishizawa, H., Inagaki, A., Suzuki, M., Ota, S., Miyamura, H., Miyazaki, J., Sekiya, T., Kurahashi, H., & Udagawa, Y. (2013). Decreased expression of apelin in placentas from severe pre-eclampsia patients. Hypertension in Pregnancy, 32(4), 410-421. https://doi.org/10.3109/10641955.2013.813535