Decreased expression of apelin in placentas from severe pre-eclampsia patients

Hiromi Inuzuka, Haruki Nishizawa, Ayaka Inagaki, Machiko Suzuki, Sayuri Ota, Hironori Miyamura, Jun Miyazaki, Takao Sekiya, Hiroki Kurahashi, Yasuhiro Udagawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: It is well documented that anti-angiogenic factors are likely to play essential roles in the etiology of pre-eclampsia. Apelin is a small peptide that may potentially act as an angiogenic factor. The expression of apelin was examined at the RNA and protein levels in this study. Methods: We compared the expression of apelin, examined using quantitative reverse-transcription polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay and immunostaining, between pre-eclamptic patients and normotensive controls. Results: Apelin messenger RNA is significantly decreased in pre-eclamptic placentas compared with normotensive pregnancies (p < 0.05). Apelin protein levels are also lower in pre-eclamptic placentas than the controls but higher in the maternal circulation in pre-eclampsia patients. Immunohistochemical signals for apelin and its receptor APJ were detected mainly in the cytoplasm of syncytiotrophoblasts in chorionic villi and trophoblast-lineage cells in the decidua of term placentas. In early gestation, stronger APJ signals were observed at the cellular membrane. Conclusions: A functional role of the apelin - APJ system is likely in early gestation, and this raises the possibility that a dysfunctional apelin - APJ system contributes to the onset of pre-eclampsia via decreased angiogenic activity in placental implantation.

Original languageEnglish
Pages (from-to)410-421
Number of pages12
JournalHypertension in Pregnancy
Volume32
Issue number4
DOIs
Publication statusPublished - 01-11-2013

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Pre-Eclampsia
Placenta
Angiogenesis Inducing Agents
Trophoblasts
Pregnancy
Chorionic Villi
Decidua
Reverse Transcription
Cytoplasm
Proteins
Western Blotting
Enzyme-Linked Immunosorbent Assay
Mothers
RNA
Polymerase Chain Reaction
Messenger RNA
Peptides
Membranes

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Obstetrics and Gynaecology

Cite this

Inuzuka, Hiromi ; Nishizawa, Haruki ; Inagaki, Ayaka ; Suzuki, Machiko ; Ota, Sayuri ; Miyamura, Hironori ; Miyazaki, Jun ; Sekiya, Takao ; Kurahashi, Hiroki ; Udagawa, Yasuhiro. / Decreased expression of apelin in placentas from severe pre-eclampsia patients. In: Hypertension in Pregnancy. 2013 ; Vol. 32, No. 4. pp. 410-421.
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Inuzuka, H, Nishizawa, H, Inagaki, A, Suzuki, M, Ota, S, Miyamura, H, Miyazaki, J, Sekiya, T, Kurahashi, H & Udagawa, Y 2013, 'Decreased expression of apelin in placentas from severe pre-eclampsia patients', Hypertension in Pregnancy, vol. 32, no. 4, pp. 410-421. https://doi.org/10.3109/10641955.2013.813535

Decreased expression of apelin in placentas from severe pre-eclampsia patients. / Inuzuka, Hiromi; Nishizawa, Haruki; Inagaki, Ayaka; Suzuki, Machiko; Ota, Sayuri; Miyamura, Hironori; Miyazaki, Jun; Sekiya, Takao; Kurahashi, Hiroki; Udagawa, Yasuhiro.

In: Hypertension in Pregnancy, Vol. 32, No. 4, 01.11.2013, p. 410-421.

Research output: Contribution to journalArticle

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T1 - Decreased expression of apelin in placentas from severe pre-eclampsia patients

AU - Inuzuka, Hiromi

AU - Nishizawa, Haruki

AU - Inagaki, Ayaka

AU - Suzuki, Machiko

AU - Ota, Sayuri

AU - Miyamura, Hironori

AU - Miyazaki, Jun

AU - Sekiya, Takao

AU - Kurahashi, Hiroki

AU - Udagawa, Yasuhiro

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Objective: It is well documented that anti-angiogenic factors are likely to play essential roles in the etiology of pre-eclampsia. Apelin is a small peptide that may potentially act as an angiogenic factor. The expression of apelin was examined at the RNA and protein levels in this study. Methods: We compared the expression of apelin, examined using quantitative reverse-transcription polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay and immunostaining, between pre-eclamptic patients and normotensive controls. Results: Apelin messenger RNA is significantly decreased in pre-eclamptic placentas compared with normotensive pregnancies (p < 0.05). Apelin protein levels are also lower in pre-eclamptic placentas than the controls but higher in the maternal circulation in pre-eclampsia patients. Immunohistochemical signals for apelin and its receptor APJ were detected mainly in the cytoplasm of syncytiotrophoblasts in chorionic villi and trophoblast-lineage cells in the decidua of term placentas. In early gestation, stronger APJ signals were observed at the cellular membrane. Conclusions: A functional role of the apelin - APJ system is likely in early gestation, and this raises the possibility that a dysfunctional apelin - APJ system contributes to the onset of pre-eclampsia via decreased angiogenic activity in placental implantation.

AB - Objective: It is well documented that anti-angiogenic factors are likely to play essential roles in the etiology of pre-eclampsia. Apelin is a small peptide that may potentially act as an angiogenic factor. The expression of apelin was examined at the RNA and protein levels in this study. Methods: We compared the expression of apelin, examined using quantitative reverse-transcription polymerase chain reaction, western blotting, enzyme-linked immunosorbent assay and immunostaining, between pre-eclamptic patients and normotensive controls. Results: Apelin messenger RNA is significantly decreased in pre-eclamptic placentas compared with normotensive pregnancies (p < 0.05). Apelin protein levels are also lower in pre-eclamptic placentas than the controls but higher in the maternal circulation in pre-eclampsia patients. Immunohistochemical signals for apelin and its receptor APJ were detected mainly in the cytoplasm of syncytiotrophoblasts in chorionic villi and trophoblast-lineage cells in the decidua of term placentas. In early gestation, stronger APJ signals were observed at the cellular membrane. Conclusions: A functional role of the apelin - APJ system is likely in early gestation, and this raises the possibility that a dysfunctional apelin - APJ system contributes to the onset of pre-eclampsia via decreased angiogenic activity in placental implantation.

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