Decreased l-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine Amino Acid Analysis

Hideaki Iizuka, Shoko Watanabe, Mayuko Koshikawa, Yurie Matsumoto, Yuki Aoyama, Hideaki Ichiba, Toshitaka Nabeshima, Takeshi Fukushima

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Abstract

It has been reported that repeated phencyclidine (PCP) treatment induces schizophrenia-like behavior in mice. l-Tryptophan (Trp) concentrations in brain tissues of control (n = 8) and PCP-treated mice (10 mg/kg/day, s.c., 14 days, n = 10) were determined using high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC method involved pre-column fluorescence derivatization with (R)-(-)-4-(N,N-dimethylaminosulfonyl)-7-(3- isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). Eight different parts of the brain, namely, the frontal cortex, nucleus accumbens, striatum, hippocampus, amygdala, thalamus, hypothalamus, and cerebellum, of both groups were investigated. A significant decrease in the l-Trp concentration in the nucleus accumbens (p = 0.024) and hippocampus (p = 0.027) was observed in PCP-treated mice, suggesting that alteration of the l-Trp metabolism might occur in these brain parts.

Original languageEnglish
Pages (from-to)8137-8141
Number of pages5
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number25
DOIs
Publication statusPublished - 01-10-2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Iizuka, H., Watanabe, S., Koshikawa, M., Matsumoto, Y., Aoyama, Y., Ichiba, H., Nabeshima, T., & Fukushima, T. (2013). Decreased l-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine Amino Acid Analysis. Analytical and Bioanalytical Chemistry, 405(25), 8137-8141. https://doi.org/10.1007/s00216-013-7010-2