Defective retrovirus insertion activates c-Ha-ras protooncogene in an MNU-induced rat mammary carcinoma

Tapan K. Bera, Tetsuya Tsukamoto, Dibyendu K. Panda, Tony Huang, Raphael C. Guzman, Soo In Hwang, Satyabrata Nandi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Endogenous retrovirus sequences are present in the genome of a wide variety of animal species. The activation of the proto oncogenes of the ras family, particularly c-Ha-ras, by either point mutation or overexpression, has been shown to be associated with a vast number of different cancers. Here we report that the insertion of a defective retrovirus in the -1 intron of rat c-Ha-ras is responsible for the activation of the gene by over 10-fold overexpression in an MNU-induced rat mammary cancer. A portion of the 3' end of the retroviral sequence is expressed as a part of the c-Ha-ras transcript in the carcinoma tissue, indicating the direct involvement of this element in the transcription of the c-Ha-ras gene. The c-Ha-ras structural gene transcribed by the promoter of the defective retroviral element can neoplastically transform the NIH 3T3 cell line upon transfection.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume248
Issue number3
DOIs
Publication statusPublished - 30-07-1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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