Degradation of human Aurora-A protein kinase is mediated by hCdh1

Sei ichi Taguchi, Kei Honda, Kazumitsu Sugiura, Akio Yamaguchi, Koichi Furukawa, Takeshi Urano

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78 Citations (Scopus)

Abstract

Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
Publication statusPublished - 22-05-2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Taguchi, S. I., Honda, K., Sugiura, K., Yamaguchi, A., Furukawa, K., & Urano, T. (2002). Degradation of human Aurora-A protein kinase is mediated by hCdh1. FEBS Letters, 519(1-3), 59-65. https://doi.org/10.1016/S0014-5793(02)02711-4