Degradation of human Aurora-A protein kinase is mediated by hCdh1

Sei ichi Taguchi, Kei Honda, Kazumitsu Sugiura, Akio Yamaguchi, Koichi Furukawa, Takeshi Urano

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
Publication statusPublished - 22-05-2002

Fingerprint

Anaphase-Promoting Complex-Cyclosome
Proteolysis
Proteasome Endopeptidase Complex
Ubiquitin
Yeast
Protein Kinases
Phosphotransferases
Association reactions
Degradation
Spindle Apparatus
Drosophila melanogaster
Saccharomyces cerevisiae
Proteins
human AURKA protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Taguchi, S. I., Honda, K., Sugiura, K., Yamaguchi, A., Furukawa, K., & Urano, T. (2002). Degradation of human Aurora-A protein kinase is mediated by hCdh1. FEBS Letters, 519(1-3), 59-65. https://doi.org/10.1016/S0014-5793(02)02711-4
Taguchi, Sei ichi ; Honda, Kei ; Sugiura, Kazumitsu ; Yamaguchi, Akio ; Furukawa, Koichi ; Urano, Takeshi. / Degradation of human Aurora-A protein kinase is mediated by hCdh1. In: FEBS Letters. 2002 ; Vol. 519, No. 1-3. pp. 59-65.
@article{7f8db638a62f4ad7a4a87a4a7a32c5ce,
title = "Degradation of human Aurora-A protein kinase is mediated by hCdh1",
abstract = "Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.",
author = "Taguchi, {Sei ichi} and Kei Honda and Kazumitsu Sugiura and Akio Yamaguchi and Koichi Furukawa and Takeshi Urano",
year = "2002",
month = "5",
day = "22",
doi = "10.1016/S0014-5793(02)02711-4",
language = "English",
volume = "519",
pages = "59--65",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "1-3",

}

Taguchi, SI, Honda, K, Sugiura, K, Yamaguchi, A, Furukawa, K & Urano, T 2002, 'Degradation of human Aurora-A protein kinase is mediated by hCdh1', FEBS Letters, vol. 519, no. 1-3, pp. 59-65. https://doi.org/10.1016/S0014-5793(02)02711-4

Degradation of human Aurora-A protein kinase is mediated by hCdh1. / Taguchi, Sei ichi; Honda, Kei; Sugiura, Kazumitsu; Yamaguchi, Akio; Furukawa, Koichi; Urano, Takeshi.

In: FEBS Letters, Vol. 519, No. 1-3, 22.05.2002, p. 59-65.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Degradation of human Aurora-A protein kinase is mediated by hCdh1

AU - Taguchi, Sei ichi

AU - Honda, Kei

AU - Sugiura, Kazumitsu

AU - Yamaguchi, Akio

AU - Furukawa, Koichi

AU - Urano, Takeshi

PY - 2002/5/22

Y1 - 2002/5/22

N2 - Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.

AB - Human Aurora-A is related to a protein kinase originally identified by its close homology to Ipl1p from Saccharomyces cerevisiae and aurora from Drosophila melanogaster, which are key regulators of the structure and function of the mitotic spindle. We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)-ubiquitin-proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.

UR - http://www.scopus.com/inward/record.url?scp=0037157197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037157197&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(02)02711-4

DO - 10.1016/S0014-5793(02)02711-4

M3 - Article

C2 - 12023018

AN - SCOPUS:0037157197

VL - 519

SP - 59

EP - 65

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1-3

ER -