Degradation of Mitochondrial Phospholipids During Experimental Cerebral Ischemia in Rats

Ichiro Nakahara, Haruhiko Kikuchi, Waro Taki, Syogo Nishi, Makoto Kito, Yasuhiro Yonekawa, Yasunobu Goto, Nobuyoshi Ogata

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Changes in content of brain mitochondrial phospholipids were examined in rats after 30 and 60 min of decapitation ischemia compared with controls, to explore the degradation of the mitochondrial membrane and its relation to dysfunction of mitochondria. Activities of respiratory functions and respiratory enzymes (cytochrome c oxidase; F0F1‐ATPase) decreased significantly during ischemia. Considerable decreases in cardiolipin and phosphatidylinositol content were observed after 60 min, and other phospholipids showed similar but nonsignificant decreases in content. The amount of polyunsaturated fatty acid chains, such as arachidonic and docosahexaenoic acids, was reduced in each phospholipid, in some cases significantly, after 30 and 60 min of ischemia in time‐dependent manners. Degradation of mitochondrial phospholipids during ischemia associated with the deterioration of mitochondrial respiratory functions suggested the significance of such changes in phospholipid content in disintegration of cellular energy metabolism during cerebral ischemia.

Original languageEnglish
Pages (from-to)839-844
Number of pages6
JournalJournal of neurochemistry
Volume57
Issue number3
DOIs
Publication statusPublished - 09-1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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