Delayed up-regulation of Zac1 and PACAP type I receptor after transient focal cerebral ischemia in mice

Frank Gillardon, Ryuji Hata, Konstantin Alexander Hossmann

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The Zac1 gene encodes a zinc finger protein that regulates both apoptosis and cell cycle arrest in vitro. Furthermore, Zac1 protein seems to trans-activate the gene encoding the type I receptor for pituitary adenylate cyclase activating polypeptide (PACAP). Northern blot analysis revealed high levels of Zac1 mRNA in the rodent brain. In the present study, we demonstrate by in situ hybridization histochemistry a progressive increase in Zac1 transcripts in the mouse brain from day 1 to day 3 following transient focal cerebral ischemia. Moreover, we observed an up-regulation of PACAP type I receptor mRNA expression showing a similar temporospatial distribution. Late induction of cell death promoting Zac1 in the post-ischemic brain may be attributed to delayed or secondary cell death. Co-induction of the type I receptor for neurotrophic PACAP however, points to a role in restorative processes. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)207-210
Number of pages4
JournalMolecular Brain Research
Volume61
Issue number1-2
DOIs
Publication statusPublished - 30-10-1998

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Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Transient Ischemic Attack
Up-Regulation
Brain
Cell Death
Messenger RNA
Zinc Fingers
Cell Cycle Checkpoints
Northern Blotting
Genes
In Situ Hybridization
Rodentia
Proteins
Apoptosis

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Gillardon, Frank ; Hata, Ryuji ; Hossmann, Konstantin Alexander. / Delayed up-regulation of Zac1 and PACAP type I receptor after transient focal cerebral ischemia in mice. In: Molecular Brain Research. 1998 ; Vol. 61, No. 1-2. pp. 207-210.
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Delayed up-regulation of Zac1 and PACAP type I receptor after transient focal cerebral ischemia in mice. / Gillardon, Frank; Hata, Ryuji; Hossmann, Konstantin Alexander.

In: Molecular Brain Research, Vol. 61, No. 1-2, 30.10.1998, p. 207-210.

Research output: Contribution to journalArticle

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AB - The Zac1 gene encodes a zinc finger protein that regulates both apoptosis and cell cycle arrest in vitro. Furthermore, Zac1 protein seems to trans-activate the gene encoding the type I receptor for pituitary adenylate cyclase activating polypeptide (PACAP). Northern blot analysis revealed high levels of Zac1 mRNA in the rodent brain. In the present study, we demonstrate by in situ hybridization histochemistry a progressive increase in Zac1 transcripts in the mouse brain from day 1 to day 3 following transient focal cerebral ischemia. Moreover, we observed an up-regulation of PACAP type I receptor mRNA expression showing a similar temporospatial distribution. Late induction of cell death promoting Zac1 in the post-ischemic brain may be attributed to delayed or secondary cell death. Co-induction of the type I receptor for neurotrophic PACAP however, points to a role in restorative processes. Copyright (C) 1998 Elsevier Science B.V.

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