TY - JOUR
T1 - Deletion of glycoprotein gM of pseudorabies virus results in attenuation for the natural host
AU - Dijkstra, Johannes M.
AU - Gerdts, Volker
AU - Klupp, Barbara G.
AU - Mettenleiter, Thomas C.
PY - 1997/9
Y1 - 1997/9
N2 - Glycoprotein M (gM) is one of the very few non-essential glycoproteins conserved throughout the herpesvirus family. Despite this conservation little is known about its function in virus replication. To test for the importance of gM in vivo in a natural virus-host system, 6-week-old piglets were intranasally infected with a gM- mutant of the alphaherpesvirus pseudorabies virus (PrV). Following infection virus excretion from the nasal mucosa was decreased ca. 100-fold compared to wild-type or revertant virus. Clinical signs were limited to transiently elevated temperature. In contrast, animals infected by wild-type or revertant virus exhibited high fever, severe respiratory symptoms and affliction of the central nervous system. Prior infection with gM- PrV conferred protection against challenge infection and animals mounted an antibody response against gM after wild-type virus infection. Thus, gM is important for efficient virus replication in vivo and deletion of gM may contribute to development of live attenuated, genetically marked vaccines.
AB - Glycoprotein M (gM) is one of the very few non-essential glycoproteins conserved throughout the herpesvirus family. Despite this conservation little is known about its function in virus replication. To test for the importance of gM in vivo in a natural virus-host system, 6-week-old piglets were intranasally infected with a gM- mutant of the alphaherpesvirus pseudorabies virus (PrV). Following infection virus excretion from the nasal mucosa was decreased ca. 100-fold compared to wild-type or revertant virus. Clinical signs were limited to transiently elevated temperature. In contrast, animals infected by wild-type or revertant virus exhibited high fever, severe respiratory symptoms and affliction of the central nervous system. Prior infection with gM- PrV conferred protection against challenge infection and animals mounted an antibody response against gM after wild-type virus infection. Thus, gM is important for efficient virus replication in vivo and deletion of gM may contribute to development of live attenuated, genetically marked vaccines.
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U2 - 10.1099/0022-1317-78-9-2147
DO - 10.1099/0022-1317-78-9-2147
M3 - Article
C2 - 9292000
AN - SCOPUS:0030931041
SN - 0022-1317
VL - 78
SP - 2147
EP - 2151
JO - Journal of General Virology
JF - Journal of General Virology
IS - 9
ER -