TY - JOUR
T1 - Delivery of an accessory signal for cell activation by exogenous phosphatidylinositol-specific phospholipase C
AU - Jamshedur Rahman, Shah Mohammad
AU - Pu, Mei yi
AU - Zhang, Yue Hua
AU - Hamaguchi, Michinari
AU - Iwamoto, Takashi
AU - Taguchi, Ryo
AU - Ikezawa, Hiroh
AU - Isobe, Ken ichi
AU - Yoshida, Tomoaki
AU - Nakashima, Izumi
N1 - Funding Information:
Acknowledgemenrs: WC thank to Dr, T. Takahashi,A ichi Cancer Center,f or donatinigm Ab, and to Dr. T. Tokuhisa.K obe University for providingc -fos cDNA, This study was supportedin part by a Grant-in-Aid from theM inistry of Education,S ciencea nd Cultureo f Japan, and The Toyota Foundation.
PY - 1992/6/1
Y1 - 1992/6/1
N2 - Digestion of phosphatidylinositol (PI) or glycosylphosphatidylinositol (GPI) anchors of membrane proteins on the external cell surface with exogenous PI-specific phospholipase C (PIPLC) from Bacillus thuringiensis was shown to transmit a signal into the thymocyte to modulate the TCR/CD3 complex-induced signal delivery for cell activation. This was demonstrated for very early protein tyrosine phosphorylation, early c-fos transcription and late DNA synthesis. For this effect preincubation of the cells with PIPLC was required, but there was no evidence of involvement of any soluble products released form the cell surface by PIPLC in the signaling, suggesting a crucial role of the membrane-bound counterpart (diacylglycerol or diradylglycerol) of the PI/GPI hydrolysate. A possible role for this accessory signal in the microorganism-linked control of the T cell receptor function is discussed.
AB - Digestion of phosphatidylinositol (PI) or glycosylphosphatidylinositol (GPI) anchors of membrane proteins on the external cell surface with exogenous PI-specific phospholipase C (PIPLC) from Bacillus thuringiensis was shown to transmit a signal into the thymocyte to modulate the TCR/CD3 complex-induced signal delivery for cell activation. This was demonstrated for very early protein tyrosine phosphorylation, early c-fos transcription and late DNA synthesis. For this effect preincubation of the cells with PIPLC was required, but there was no evidence of involvement of any soluble products released form the cell surface by PIPLC in the signaling, suggesting a crucial role of the membrane-bound counterpart (diacylglycerol or diradylglycerol) of the PI/GPI hydrolysate. A possible role for this accessory signal in the microorganism-linked control of the T cell receptor function is discussed.
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U2 - 10.1016/0014-5793(92)80517-K
DO - 10.1016/0014-5793(92)80517-K
M3 - Article
C2 - 1535048
AN - SCOPUS:0026582410
SN - 0014-5793
VL - 303
SP - 193
EP - 196
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -