Delivery of an accessory signal for cell activation by exogenous phosphatidylinositol-specific phospholipase C

Shah Mohammad Jamshedur Rahman, Mei yi Pu, Yue Hua Zhang, Michinari Hamaguchi, Takashi Iwamoto, Ryo Taguchi, Hiroh Ikezawa, Ken ichi Isobe, Tomoaki Yoshida, Izumi Nakashima

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Digestion of phosphatidylinositol (PI) or glycosylphosphatidylinositol (GPI) anchors of membrane proteins on the external cell surface with exogenous PI-specific phospholipase C (PIPLC) from Bacillus thuringiensis was shown to transmit a signal into the thymocyte to modulate the TCR/CD3 complex-induced signal delivery for cell activation. This was demonstrated for very early protein tyrosine phosphorylation, early c-fos transcription and late DNA synthesis. For this effect preincubation of the cells with PIPLC was required, but there was no evidence of involvement of any soluble products released form the cell surface by PIPLC in the signaling, suggesting a crucial role of the membrane-bound counterpart (diacylglycerol or diradylglycerol) of the PI/GPI hydrolysate. A possible role for this accessory signal in the microorganism-linked control of the T cell receptor function is discussed.

Original languageEnglish
Pages (from-to)193-196
Number of pages4
JournalFEBS Letters
Volume303
Issue number2-3
DOIs
Publication statusPublished - 01-06-1992
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Delivery of an accessory signal for cell activation by exogenous phosphatidylinositol-specific phospholipase C'. Together they form a unique fingerprint.

Cite this