Demonstration of lipid A-binding proteins on murine lymphoma cells using R-mutant Gram-negative bacteria as a detector

I. Nakashima, T. Kubota, Y. Zhang, T. Yoshida, R. Inagi, K. Isobe, F. Nagase, M. Ohta, N. Kato, T. Yokochi

Research output: Contribution to journalArticle

Abstract

Some R-mutant Escherichia coli and Salmonella heavily adhered to murine lymphoma cells of B cell and T cell lineages. This adhesion was primarily mediated by membrane-localized proteins on tumor cells, which bind the polymyxin B-reactive hydrophilic structure of lipid A on bacteria. SDS-PAGE analysis of tumor cell membranes showed that proteins or glycoproteins of MW = around 45Kd, 25-35Kd and around 15Kd preferentially bind lipid A. Various lymphoma cell lines binding the bacteria at different levels possessed lipid A-binding proteins of slightly different compositions. We conclude that lymphoma cells carry not a single but a group of lipid A-binding proteins in their membranes.

Original languageEnglish
Pages (from-to)87-94
Number of pages8
JournalAPMIS
Volume98
Issue number1
DOIs
Publication statusPublished - 1990

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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    Nakashima, I., Kubota, T., Zhang, Y., Yoshida, T., Inagi, R., Isobe, K., Nagase, F., Ohta, M., Kato, N., & Yokochi, T. (1990). Demonstration of lipid A-binding proteins on murine lymphoma cells using R-mutant Gram-negative bacteria as a detector. APMIS, 98(1), 87-94. https://doi.org/10.1111/j.1699-0463.1990.tb01006.x