Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis

Yoshinori Ito; Yoshiki Kawamura; Seiko Iwata; Jun ichi Kawada; Tetsushi Yoshikawa; Hiroshi Kimura

doi:10.1002/pbc.24319

Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis

Yoshinori Ito, Yoshiki Kawamura, Seiko Iwata, Jun ichi Kawada, Tetsushi Yoshikawa, Hiroshi Kimura

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Epstein-Barr virus (EBV) is the most common infectious cause of non-genetic hemophagocytic lymphohistiocytosis (HLH). To investigate EBV-infected lymphocytes and immune dysfunction in EBV-associated HLH, blood samples from a 6-year-old boy were longitudinally analyzed using molecular techniques. EBV-positive lymphocytes were detected as CD5⁺, CD8⁺, and/or HLA DR⁺ lymphocytes on Day 25 of the disease, mostly disappearing thereafter. CD8⁺ cells specific for lytic antigen BRLF1 were detected, but cells specific for latent antigens EBNA3 and LMP2 were not. EBV genes EBNA1, LMP1, LMP2, EBER1, BARTs were detected, suggesting a latency type II gene expression pattern in this case.

Original language	English
Pages (from-to)	326-328
Number of pages	3
Journal	Pediatric Blood and Cancer
Volume	60
Issue number	2
DOIs	https://doi.org/10.1002/pbc.24319
Publication status	Published - 02-2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pediatrics, Perinatology, and Child Health
Hematology
Oncology

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10.1002/pbc.24319

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title = "Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis",

abstract = "Epstein-Barr virus (EBV) is the most common infectious cause of non-genetic hemophagocytic lymphohistiocytosis (HLH). To investigate EBV-infected lymphocytes and immune dysfunction in EBV-associated HLH, blood samples from a 6-year-old boy were longitudinally analyzed using molecular techniques. EBV-positive lymphocytes were detected as CD5+, CD8+, and/or HLA DR+ lymphocytes on Day 25 of the disease, mostly disappearing thereafter. CD8+ cells specific for lytic antigen BRLF1 were detected, but cells specific for latent antigens EBNA3 and LMP2 were not. EBV genes EBNA1, LMP1, LMP2, EBER1, BARTs were detected, suggesting a latency type II gene expression pattern in this case.",

author = "Yoshinori Ito and Yoshiki Kawamura and Seiko Iwata and Kawada, \{Jun ichi\} and Tetsushi Yoshikawa and Hiroshi Kimura",

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journal = "Pediatric Blood and Cancer",

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T1 - Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis

AU - Ito, Yoshinori

AU - Kawamura, Yoshiki

AU - Iwata, Seiko

AU - Kawada, Jun ichi

AU - Yoshikawa, Tetsushi

AU - Kimura, Hiroshi

PY - 2013/2

Y1 - 2013/2

N2 - Epstein-Barr virus (EBV) is the most common infectious cause of non-genetic hemophagocytic lymphohistiocytosis (HLH). To investigate EBV-infected lymphocytes and immune dysfunction in EBV-associated HLH, blood samples from a 6-year-old boy were longitudinally analyzed using molecular techniques. EBV-positive lymphocytes were detected as CD5+, CD8+, and/or HLA DR+ lymphocytes on Day 25 of the disease, mostly disappearing thereafter. CD8+ cells specific for lytic antigen BRLF1 were detected, but cells specific for latent antigens EBNA3 and LMP2 were not. EBV genes EBNA1, LMP1, LMP2, EBER1, BARTs were detected, suggesting a latency type II gene expression pattern in this case.

AB - Epstein-Barr virus (EBV) is the most common infectious cause of non-genetic hemophagocytic lymphohistiocytosis (HLH). To investigate EBV-infected lymphocytes and immune dysfunction in EBV-associated HLH, blood samples from a 6-year-old boy were longitudinally analyzed using molecular techniques. EBV-positive lymphocytes were detected as CD5+, CD8+, and/or HLA DR+ lymphocytes on Day 25 of the disease, mostly disappearing thereafter. CD8+ cells specific for lytic antigen BRLF1 were detected, but cells specific for latent antigens EBNA3 and LMP2 were not. EBV genes EBNA1, LMP1, LMP2, EBER1, BARTs were detected, suggesting a latency type II gene expression pattern in this case.

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Demonstration of type II latency in T lymphocytes of Epstein-Barr Virus-associated hemophagocytic lymphohistiocytosis

Abstract

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