Detection and genotyping of GBV-C/HGV variants in China

Ling Lu; Mun Hon Ng; Bei Ping Zhou; Hong Tao Luo; Tatsunori Nakano; Betty H. Robertson; Stanley W.K. Im

doi:10.1016/S0168-1702(00)00231-8

Detection and genotyping of GBV-C/HGV variants in China

Ling Lu, Mun Hon Ng, Bei Ping Zhou, Hong Tao Luo, Tatsunori Nakano, Betty H. Robertson, Stanley W.K. Im

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We detected GBV-C/HGV sequences in the sera from 64 out of a total of 324 subjects in the south of China. In agreement with findings of others, we noted an especially high rate of infection among intravenous drug addicts and patients with chronic hepatitis C virus infection. The detection was achieved by nested PCR to amplify the 5′ noncoding region (5′NCR) of the viral genome. Sequence analysis of the resulting 234 bp product revealed a total of 26 different sequences of which 25 were found to belong to the genotype G3, which is the most prevalent genotypes among Asian isolates, and one belonged to genotype G1, common among African isolates. The sequence divergence between the genotypes was largely clustered in a short variable region (V2) within the 5′NCR, and we showed that genotyping may be achieved equally well by analysis of this variable region as by the more detail analysis of the entire 5′NCR or of the entire viral genome.

Original language	English
Pages (from-to)	131-144
Number of pages	14
Journal	Virus Research
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/S0168-1702(00)00231-8
Publication status	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Cancer Research
Virology
Infectious Diseases

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10.1016/S0168-1702(00)00231-8

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title = "Detection and genotyping of GBV-C/HGV variants in China",

abstract = "We detected GBV-C/HGV sequences in the sera from 64 out of a total of 324 subjects in the south of China. In agreement with findings of others, we noted an especially high rate of infection among intravenous drug addicts and patients with chronic hepatitis C virus infection. The detection was achieved by nested PCR to amplify the 5′ noncoding region (5′NCR) of the viral genome. Sequence analysis of the resulting 234 bp product revealed a total of 26 different sequences of which 25 were found to belong to the genotype G3, which is the most prevalent genotypes among Asian isolates, and one belonged to genotype G1, common among African isolates. The sequence divergence between the genotypes was largely clustered in a short variable region (V2) within the 5′NCR, and we showed that genotyping may be achieved equally well by analysis of this variable region as by the more detail analysis of the entire 5′NCR or of the entire viral genome.",

author = "Ling Lu and Ng, {Mun Hon} and Zhou, {Bei Ping} and Luo, {Hong Tao} and Tatsunori Nakano and Robertson, {Betty H.} and Im, {Stanley W.K.}",

note = "Funding Information: This work was supported by the Committee on Research and Conference Grants (CRCG) of The University of Hong Kong, the Industrial Support Fund from the industrial department of the government of Hong Kong Special Administrative Region, and a special grant for high-technology development in the year 1996–1997 from the Commission of Science and Technology, Guangdong Province, the People's Republic of China. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "2001",

doi = "10.1016/S0168-1702(00)00231-8",

language = "English",

volume = "73",

pages = "131--144",

journal = "Virus Research",

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T1 - Detection and genotyping of GBV-C/HGV variants in China

AU - Lu, Ling

AU - Ng, Mun Hon

AU - Zhou, Bei Ping

AU - Luo, Hong Tao

AU - Nakano, Tatsunori

AU - Robertson, Betty H.

AU - Im, Stanley W.K.

N1 - Funding Information: This work was supported by the Committee on Research and Conference Grants (CRCG) of The University of Hong Kong, the Industrial Support Fund from the industrial department of the government of Hong Kong Special Administrative Region, and a special grant for high-technology development in the year 1996–1997 from the Commission of Science and Technology, Guangdong Province, the People's Republic of China. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2001

Y1 - 2001

N2 - We detected GBV-C/HGV sequences in the sera from 64 out of a total of 324 subjects in the south of China. In agreement with findings of others, we noted an especially high rate of infection among intravenous drug addicts and patients with chronic hepatitis C virus infection. The detection was achieved by nested PCR to amplify the 5′ noncoding region (5′NCR) of the viral genome. Sequence analysis of the resulting 234 bp product revealed a total of 26 different sequences of which 25 were found to belong to the genotype G3, which is the most prevalent genotypes among Asian isolates, and one belonged to genotype G1, common among African isolates. The sequence divergence between the genotypes was largely clustered in a short variable region (V2) within the 5′NCR, and we showed that genotyping may be achieved equally well by analysis of this variable region as by the more detail analysis of the entire 5′NCR or of the entire viral genome.

AB - We detected GBV-C/HGV sequences in the sera from 64 out of a total of 324 subjects in the south of China. In agreement with findings of others, we noted an especially high rate of infection among intravenous drug addicts and patients with chronic hepatitis C virus infection. The detection was achieved by nested PCR to amplify the 5′ noncoding region (5′NCR) of the viral genome. Sequence analysis of the resulting 234 bp product revealed a total of 26 different sequences of which 25 were found to belong to the genotype G3, which is the most prevalent genotypes among Asian isolates, and one belonged to genotype G1, common among African isolates. The sequence divergence between the genotypes was largely clustered in a short variable region (V2) within the 5′NCR, and we showed that genotyping may be achieved equally well by analysis of this variable region as by the more detail analysis of the entire 5′NCR or of the entire viral genome.

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JO - Virus Research

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Detection and genotyping of GBV-C/HGV variants in China

Abstract

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