Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification

Tetsushi Yoshikawa, Masaru Ihira, Shiho Akimoto, Chie Usui, Fumi Miyake, Sadao Suga, Yoshihiko Enomoto, Ryota Suzuki, Yukihiro Nishiyama, Yoshizo Asano

Research output: Contribution to journalArticle

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Abstract

The reliability of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this study. Although a LAMP product was detected in HHV-7 DNA, neither HHV-6 nor human cytomegalovirus DNA produced a product. When agarose gel electrophoresis was used for the detection of LAMP products, the sensitivity of a 30-min HHV-7 LAMP reaction reached 250 copies/tube. The use of turbidity for the detection of the LAMP products gave a sensitivity of 500 and 250 copies/tube for 30- and 60-min reactions, respectively. Following these initial validation studies, clinical samples collected from two patients with primary HHV-7 infections were examined by HHV-7 LAMP. By use of agarose gel electrophoresis, HHV-7 LAMP products could be detected in acute-phase plasma samples but no LAMP product was detectable in convalescent-phase plasma samples from either patient. Since a turbidity assay is less sensitive than agarose gel electrophoresis, no HHV-7 LAMP product could be detected in plasma samples after a 30-min LAMP reaction. After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples.

Original languageEnglish
Pages (from-to)1348-1352
Number of pages5
JournalJournal of clinical microbiology
Volume42
Issue number3
DOIs
Publication statusPublished - 01-03-2004

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Human Herpesvirus 7
DNA
Agar Gel Electrophoresis
Human Herpesvirus 6
Herpesviridae Infections
Validation Studies
Cytomegalovirus

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Yoshikawa, Tetsushi ; Ihira, Masaru ; Akimoto, Shiho ; Usui, Chie ; Miyake, Fumi ; Suga, Sadao ; Enomoto, Yoshihiko ; Suzuki, Ryota ; Nishiyama, Yukihiro ; Asano, Yoshizo. / Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification. In: Journal of clinical microbiology. 2004 ; Vol. 42, No. 3. pp. 1348-1352.
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Yoshikawa, T, Ihira, M, Akimoto, S, Usui, C, Miyake, F, Suga, S, Enomoto, Y, Suzuki, R, Nishiyama, Y & Asano, Y 2004, 'Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification', Journal of clinical microbiology, vol. 42, no. 3, pp. 1348-1352. https://doi.org/10.1128/JCM.42.3.1348-1352.2004

Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification. / Yoshikawa, Tetsushi; Ihira, Masaru; Akimoto, Shiho; Usui, Chie; Miyake, Fumi; Suga, Sadao; Enomoto, Yoshihiko; Suzuki, Ryota; Nishiyama, Yukihiro; Asano, Yoshizo.

In: Journal of clinical microbiology, Vol. 42, No. 3, 01.03.2004, p. 1348-1352.

Research output: Contribution to journalArticle

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T1 - Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification

AU - Yoshikawa, Tetsushi

AU - Ihira, Masaru

AU - Akimoto, Shiho

AU - Usui, Chie

AU - Miyake, Fumi

AU - Suga, Sadao

AU - Enomoto, Yoshihiko

AU - Suzuki, Ryota

AU - Nishiyama, Yukihiro

AU - Asano, Yoshizo

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AB - The reliability of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this study. Although a LAMP product was detected in HHV-7 DNA, neither HHV-6 nor human cytomegalovirus DNA produced a product. When agarose gel electrophoresis was used for the detection of LAMP products, the sensitivity of a 30-min HHV-7 LAMP reaction reached 250 copies/tube. The use of turbidity for the detection of the LAMP products gave a sensitivity of 500 and 250 copies/tube for 30- and 60-min reactions, respectively. Following these initial validation studies, clinical samples collected from two patients with primary HHV-7 infections were examined by HHV-7 LAMP. By use of agarose gel electrophoresis, HHV-7 LAMP products could be detected in acute-phase plasma samples but no LAMP product was detectable in convalescent-phase plasma samples from either patient. Since a turbidity assay is less sensitive than agarose gel electrophoresis, no HHV-7 LAMP product could be detected in plasma samples after a 30-min LAMP reaction. After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples.

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