Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification

Tetsushi Yoshikawa, Masaru Ihira, Shiho Akimoto, Chie Usui, Fumi Miyake, Sadao Suga, Yoshihiko Enomoto, Ryota Suzuki, Yukihiro Nishiyama, Yoshizo Asano

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Abstract

The reliability of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this study. Although a LAMP product was detected in HHV-7 DNA, neither HHV-6 nor human cytomegalovirus DNA produced a product. When agarose gel electrophoresis was used for the detection of LAMP products, the sensitivity of a 30-min HHV-7 LAMP reaction reached 250 copies/tube. The use of turbidity for the detection of the LAMP products gave a sensitivity of 500 and 250 copies/tube for 30- and 60-min reactions, respectively. Following these initial validation studies, clinical samples collected from two patients with primary HHV-7 infections were examined by HHV-7 LAMP. By use of agarose gel electrophoresis, HHV-7 LAMP products could be detected in acute-phase plasma samples but no LAMP product was detectable in convalescent-phase plasma samples from either patient. Since a turbidity assay is less sensitive than agarose gel electrophoresis, no HHV-7 LAMP product could be detected in plasma samples after a 30-min LAMP reaction. After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples.

Original languageEnglish
Pages (from-to)1348-1352
Number of pages5
JournalJournal of clinical microbiology
Volume42
Issue number3
DOIs
Publication statusPublished - 01-03-2004

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All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Yoshikawa, T., Ihira, M., Akimoto, S., Usui, C., Miyake, F., Suga, S., Enomoto, Y., Suzuki, R., Nishiyama, Y., & Asano, Y. (2004). Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification. Journal of clinical microbiology, 42(3), 1348-1352. https://doi.org/10.1128/JCM.42.3.1348-1352.2004