Detection of new anti-neutral glycosphingolipids antibodies and their effects on Trk neurotrophin receptors

T. Mihara; A. Ueda; M. Hirayama; T. Takeuchi; S. Yoshida; K. Naito; H. Yamamoto; T. Mutoh

doi:10.1016/j.febslet.2006.08.013

Detection of new anti-neutral glycosphingolipids antibodies and their effects on Trk neurotrophin receptors

T. Mihara, A. Ueda, M. Hirayama, T. Takeuchi, S. Yoshida, K. Naito, H. Yamamoto, T. Mutoh

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23 Citations (Scopus)

Abstract

We screened sera from patients with various neurological disorders for the presence of anti-neutral glycosphingolipids antibodies and only found them in sera from relapsing polychondritis with limbic encephalitis patients. Neutral glycosphingolipids are resident in membrane lipid rafts where high affinity nerve growth factor (NGF) receptor, Trk is co-localized. Therefore, we examined whether these antibodies influence the action of NGF in NGF-responsive cells. The results strongly suggest that these antibodies enhance NGF-induced Trk autophosphorylation and neurite outgrowth as well as neurofilament M expression. These data strongly indicate that these anti-neutral glycosphingolipids antibodies have an functional impact on NGF-Trk-mediated intracellular signal transduction pathway.

Original language	English
Pages (from-to)	4991-4995
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2006.08.013
Publication status	Published - 18-09-2006

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2006.08.013

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title = "Detection of new anti-neutral glycosphingolipids antibodies and their effects on Trk neurotrophin receptors",

abstract = "We screened sera from patients with various neurological disorders for the presence of anti-neutral glycosphingolipids antibodies and only found them in sera from relapsing polychondritis with limbic encephalitis patients. Neutral glycosphingolipids are resident in membrane lipid rafts where high affinity nerve growth factor (NGF) receptor, Trk is co-localized. Therefore, we examined whether these antibodies influence the action of NGF in NGF-responsive cells. The results strongly suggest that these antibodies enhance NGF-induced Trk autophosphorylation and neurite outgrowth as well as neurofilament M expression. These data strongly indicate that these anti-neutral glycosphingolipids antibodies have an functional impact on NGF-Trk-mediated intracellular signal transduction pathway.",

author = "T. Mihara and A. Ueda and M. Hirayama and T. Takeuchi and S. Yoshida and K. Naito and H. Yamamoto and T. Mutoh",

note = "Funding Information: The present study was partly supported by the grant-in-aid on Center of Excellence (COE), a priority area on Functional Glycomics, and High-Tech research from the Ministry of education, culture, sports, science and technology of Japan. We deeply thank Dr. Y. Hirabayashi for his kind supply of purified GlcCer and chemically synthesized pure GlcCer.",

year = "2006",

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doi = "10.1016/j.febslet.2006.08.013",

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T1 - Detection of new anti-neutral glycosphingolipids antibodies and their effects on Trk neurotrophin receptors

AU - Mihara, T.

AU - Ueda, A.

AU - Hirayama, M.

AU - Takeuchi, T.

AU - Yoshida, S.

AU - Naito, K.

AU - Yamamoto, H.

AU - Mutoh, T.

N1 - Funding Information: The present study was partly supported by the grant-in-aid on Center of Excellence (COE), a priority area on Functional Glycomics, and High-Tech research from the Ministry of education, culture, sports, science and technology of Japan. We deeply thank Dr. Y. Hirabayashi for his kind supply of purified GlcCer and chemically synthesized pure GlcCer.

PY - 2006/9/18

Y1 - 2006/9/18

N2 - We screened sera from patients with various neurological disorders for the presence of anti-neutral glycosphingolipids antibodies and only found them in sera from relapsing polychondritis with limbic encephalitis patients. Neutral glycosphingolipids are resident in membrane lipid rafts where high affinity nerve growth factor (NGF) receptor, Trk is co-localized. Therefore, we examined whether these antibodies influence the action of NGF in NGF-responsive cells. The results strongly suggest that these antibodies enhance NGF-induced Trk autophosphorylation and neurite outgrowth as well as neurofilament M expression. These data strongly indicate that these anti-neutral glycosphingolipids antibodies have an functional impact on NGF-Trk-mediated intracellular signal transduction pathway.

AB - We screened sera from patients with various neurological disorders for the presence of anti-neutral glycosphingolipids antibodies and only found them in sera from relapsing polychondritis with limbic encephalitis patients. Neutral glycosphingolipids are resident in membrane lipid rafts where high affinity nerve growth factor (NGF) receptor, Trk is co-localized. Therefore, we examined whether these antibodies influence the action of NGF in NGF-responsive cells. The results strongly suggest that these antibodies enhance NGF-induced Trk autophosphorylation and neurite outgrowth as well as neurofilament M expression. These data strongly indicate that these anti-neutral glycosphingolipids antibodies have an functional impact on NGF-Trk-mediated intracellular signal transduction pathway.

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Detection of new anti-neutral glycosphingolipids antibodies and their effects on Trk neurotrophin receptors

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