Detection of norovirus-like particles with an external force-assisted near-field illumination biosensor

Masato Yasuura, Haruko Shirato, Kyoko Moriguchi, Makoto Fujimaki

Research output: Contribution to journalArticle

Abstract

Optical near-field enhancement at a surface where a total internal reflection occurs is an effective phenomenon that can enhance optical signals from markers conjugated with biological materials on the surface. The enhanced signal can be an effective tool to detect trace amounts of a biological substance. An external force-assisted near-field illumination biosensor is based on a sandwich assay that uses magnetic particles instead of a sensor surface to capture the targets. This sensing method detects light signals from targets sandwiched between magnetic particles and optical markers. The signal of the sandwiched target can be easily distinguished from optical noise by observing the movement of the signal induced by an external magnetic field. In this study, we improved the detection ability by using 60 nm ∅ gold nanoparticles as markers. Norovirus virus-like particles were added to a buffer or a contaminated water sample and successfully detected using this system.

Original languageEnglish
Article number071005
JournalJapanese Journal of Applied Physics
Volume58
Issue number7
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

bioinstrumentation
Biosensors
near fields
Lighting
illumination
markers
Viruses
Biological materials
Assays
viruses
Gold
Magnetic fields
Nanoparticles
optical communication
buffers
Sensors
gold
nanoparticles
augmentation
Water

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

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Detection of norovirus-like particles with an external force-assisted near-field illumination biosensor. / Yasuura, Masato; Shirato, Haruko; Moriguchi, Kyoko; Fujimaki, Makoto.

In: Japanese Journal of Applied Physics, Vol. 58, No. 7, 071005, 01.01.2019.

Research output: Contribution to journalArticle

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