TY - JOUR
T1 - Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction
AU - Tamura, Yasutsugu
AU - Furukawa, Kazuhiro
AU - Yoshimoto, Rei
AU - Kawai, Yuto
AU - Yoshida, Minoru
AU - Tsuneda, Satoshi
AU - Ito, Yoshihiro
AU - Abe, Hiroshi
N1 - Funding Information:
K.F., Y.I., and H.A. were financially supported by JSPS, by MEXT, and by MEXT and NEDO, respectively. We thank the RIKEN BSI Research Resources Center for the mass spectrum analysis.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.
AB - RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.
UR - http://www.scopus.com/inward/record.url?scp=84869091977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869091977&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2012.09.033
DO - 10.1016/j.bmcl.2012.09.033
M3 - Article
C2 - 23072867
AN - SCOPUS:84869091977
SN - 0960-894X
VL - 22
SP - 7248
EP - 7251
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 23
ER -