Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction

Yasutsugu Tamura, Kazuhiro Furukawa, Rei Yoshimoto, Yuto Kawai, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito, Hiroshi Abe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

RNA splicing is an important target for basic research of disease mechanisms and for drug discovery. Here, we report a new method for analysis of the in vitro RNA splicing process that produces fluorescence using a reduction-triggered fluorescence (RETF) probe. The fluorescence signal is produced only when the two probes bind side-by-side with a specific RNA target. Precursor messenger RNA and mature messenger RNA originating from the chicken δ-crystallin (CDC) gene were successfully discriminated in solution using an RETF probe with the assistance of helper oligonucleotide strands. Also, we successfully applied RETF probes to the detection of emerging mature mRNA in an in vitro splicing process.

Original languageEnglish
Pages (from-to)7248-7251
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume22
Issue number23
DOIs
Publication statusPublished - 01-12-2012

Fingerprint

RNA Precursors
Fluorescence
RNA
RNA Splicing
Messenger RNA
Crystallins
Drug Discovery
Centers for Disease Control and Prevention (U.S.)
Oligonucleotides
Chickens
Genes
In Vitro Techniques
Research

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Tamura, Yasutsugu ; Furukawa, Kazuhiro ; Yoshimoto, Rei ; Kawai, Yuto ; Yoshida, Minoru ; Tsuneda, Satoshi ; Ito, Yoshihiro ; Abe, Hiroshi. / Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction. In: Bioorganic and Medicinal Chemistry Letters. 2012 ; Vol. 22, No. 23. pp. 7248-7251.
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Detection of pre-mRNA splicing in vitro by an RNA-templated fluorogenic reaction. / Tamura, Yasutsugu; Furukawa, Kazuhiro; Yoshimoto, Rei; Kawai, Yuto; Yoshida, Minoru; Tsuneda, Satoshi; Ito, Yoshihiro; Abe, Hiroshi.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 22, No. 23, 01.12.2012, p. 7248-7251.

Research output: Contribution to journalArticle

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