TY - JOUR
T1 - Detection of Ret homodimers in MEN 2A-associated pheochromocytomas
AU - Wada, Masaki
AU - Asai, Naoya
AU - Tsuzuki, Toyonori
AU - Maruyama, Shoichi
AU - Ohiwa, Mikinao
AU - Imai, Tsuneo
AU - Funahashi, Hiroomi
AU - Takagi, Hiroshi
AU - Takahashi, Masahide
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research and for Cancer Research from the Ministry of Education, Science, and Culture of Japan and by a grant from the Nitto Foundation.
PY - 1996/1/17
Y1 - 1996/1/17
N2 - Using transfection of NIH 3T3 cells, we have recently demonstrated that multiple endocrine neoplasia (MEN) 2A mutations activate the c-Ret protein by inducing its disulfide-linked homodimerization on the cell surface. To investigate whether the homodimers are present in original tumors, the expression of the c-Ret protein was analyzed in eight sporadic and two MEN 2A-associated pheochromocytomas, the latter two of which contained mutations in cysteine 618 or 634 of Ret. The c-Ret protein was expressed at variable levels in all pheochromocytomas examined. By labeling the c-Ret protein immunoprecipitated from tumor tissues with [γ-32P]ATP in vitro, its homodimers were detected in pheochromocytomas from MEN 2A patients but not in a sporadic tumor. This result represents the first demonstration of Ret homodimers in original tumors.
AB - Using transfection of NIH 3T3 cells, we have recently demonstrated that multiple endocrine neoplasia (MEN) 2A mutations activate the c-Ret protein by inducing its disulfide-linked homodimerization on the cell surface. To investigate whether the homodimers are present in original tumors, the expression of the c-Ret protein was analyzed in eight sporadic and two MEN 2A-associated pheochromocytomas, the latter two of which contained mutations in cysteine 618 or 634 of Ret. The c-Ret protein was expressed at variable levels in all pheochromocytomas examined. By labeling the c-Ret protein immunoprecipitated from tumor tissues with [γ-32P]ATP in vitro, its homodimers were detected in pheochromocytomas from MEN 2A patients but not in a sporadic tumor. This result represents the first demonstration of Ret homodimers in original tumors.
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U2 - 10.1006/bbrc.1996.0107
DO - 10.1006/bbrc.1996.0107
M3 - Article
C2 - 8561803
AN - SCOPUS:0030045614
SN - 0006-291X
VL - 218
SP - 606
EP - 609
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -