TY - JOUR
T1 - Determination of 3-hydroxyisovalerylcarnitine and other acylcarnitine levels using liquid chromatography-tandem mass spectrometry in serum and urine of a patient with multiple carboxylase deficiency
AU - Maeda, Yasuhiro
AU - Ito, Tetsuya
AU - Ohmi, Hironori
AU - Yokoi, Kyoko
AU - Nakajima, Yoko
AU - Ueta, Akihito
AU - Kurono, Yukihisa
AU - Togari, Hajime
AU - Sugiyama, Naruji
N1 - Funding Information:
This research was supported by Grant-in-Aid for Research in Nagoya City University.
PY - 2008/7/15
Y1 - 2008/7/15
N2 - Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.
AB - Due to its increased concentration in blood, 3-hydroxyisovalerylcarnitine (C5OH-I) is an important indicator for the diagnosis of organic acidemias in newborns. However, C5OH-I has not been used as a standard in tandem mass spectrometric (MS/MS) assays because its isolation is difficult. We developed a new synthesis of C5OH-I and investigated its behavior by MS/MS. A method using the multiple reaction monitoring (MRM) mode of MS/MS with HPLC was developed which provides high accuracy, precision and reproducibility. Acylcarnitine profiles in the serum and urine of a patient with multiple carboxylase deficiency (MCD) showed increased levels compared to a healthy patient.
UR - http://www.scopus.com/inward/record.url?scp=47249124537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47249124537&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2007.11.037
DO - 10.1016/j.jchromb.2007.11.037
M3 - Article
C2 - 18088573
AN - SCOPUS:47249124537
SN - 1570-0232
VL - 870
SP - 154
EP - 159
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -