Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi, Yasuo Tokushima, Kiyomi Oishi, Yasuyuki Takahashi, Satoshi Shiojiri, Shigenobu Nakamura, Seigo Tanaka, Ryoji Kodaira, Hirataka Ito

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

Original languageEnglish
Pages (from-to)1453-1459
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number3
DOIs
Publication statusPublished - 14-02-1990

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Cerebrospinal fluid
Amyloid beta-Protein Precursor
Trypsin
Cerebrospinal Fluid
Alzheimer Disease
Peptide Hydrolases
Enzyme-Linked Immunosorbent Assay
Trypsin Inhibitors
Antibodies
Multi-Infarct Dementia
Immunosorbents
Denaturation
Assays
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kitaguchi, Nobuya ; Tokushima, Yasuo ; Oishi, Kiyomi ; Takahashi, Yasuyuki ; Shiojiri, Satoshi ; Nakamura, Shigenobu ; Tanaka, Seigo ; Kodaira, Ryoji ; Ito, Hirataka. / Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 166, No. 3. pp. 1453-1459.
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Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. / Kitaguchi, Nobuya; Tokushima, Yasuo; Oishi, Kiyomi; Takahashi, Yasuyuki; Shiojiri, Satoshi; Nakamura, Shigenobu; Tanaka, Seigo; Kodaira, Ryoji; Ito, Hirataka.

In: Biochemical and Biophysical Research Communications, Vol. 166, No. 3, 14.02.1990, p. 1453-1459.

Research output: Contribution to journalArticle

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