Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi; Yasuo Tokushima; Kiyomi Oishi; Yasuyuki Takahashi; Satoshi Shiojiri; Shigenobu Nakamura; Seigo Tanaka; Ryoji Kodaira; Hirataka Ito

doi:10.1016/0006-291X(90)91030-V

Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi, Yasuo Tokushima, Kiyomi Oishi, Yasuyuki Takahashi, Satoshi Shiojiri, Shigenobu Nakamura, Seigo Tanaka, Ryoji Kodaira, Hirataka Ito

Faculty of Clinical Engineering

Research output: Contribution to journal › Article

39 Citations (Scopus)

Abstract

β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

Original language	English
Pages (from-to)	1453-1459
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	166
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)91030-V
Publication status	Published - 14-02-1990

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kitaguchi, N., Tokushima, Y., Oishi, K., Takahashi, Y., Shiojiri, S., Nakamura, S., Tanaka, S., Kodaira, R., & Ito, H. (1990). Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. Biochemical and Biophysical Research Communications, 166(3), 1453-1459. https://doi.org/10.1016/0006-291X(90)91030-V

Kitaguchi, Nobuya ; Tokushima, Yasuo ; Oishi, Kiyomi ; Takahashi, Yasuyuki ; Shiojiri, Satoshi ; Nakamura, Shigenobu ; Tanaka, Seigo ; Kodaira, Ryoji ; Ito, Hirataka. / Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 166, No. 3. pp. 1453-1459.

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abstract = "β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.",

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Kitaguchi, N, Tokushima, Y, Oishi, K, Takahashi, Y, Shiojiri, S, Nakamura, S, Tanaka, S, Kodaira, R & Ito, H 1990, 'Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA', Biochemical and Biophysical Research Communications, vol. 166, no. 3, pp. 1453-1459. https://doi.org/10.1016/0006-291X(90)91030-V

Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. / Kitaguchi, Nobuya; Tokushima, Yasuo; Oishi, Kiyomi; Takahashi, Yasuyuki; Shiojiri, Satoshi; Nakamura, Shigenobu; Tanaka, Seigo; Kodaira, Ryoji; Ito, Hirataka.

In: Biochemical and Biophysical Research Communications, Vol. 166, No. 3, 14.02.1990, p. 1453-1459.

Research output: Contribution to journal › Article

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Kitaguchi N, Tokushima Y, Oishi K, Takahashi Y, Shiojiri S, Nakamura S et al. Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. Biochemical and Biophysical Research Communications. 1990 Feb 14;166(3):1453-1459. https://doi.org/10.1016/0006-291X(90)91030-V