Abstract
β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.
| Original language | English |
|---|---|
| Pages (from-to) | 1453-1459 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 166 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 14-02-1990 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. / Kitaguchi, Nobuya; Tokushima, Yasuo; Oishi, Kiyomi; Takahashi, Yasuyuki; Shiojiri, Satoshi; Nakamura, Shigenobu; Tanaka, Seigo; Kodaira, Ryoji; Ito, Hirataka.
In: Biochemical and Biophysical Research Communications, Vol. 166, No. 3, 14.02.1990, p. 1453-1459.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA
AU - Kitaguchi, Nobuya
AU - Tokushima, Yasuo
AU - Oishi, Kiyomi
AU - Takahashi, Yasuyuki
AU - Shiojiri, Satoshi
AU - Nakamura, Shigenobu
AU - Tanaka, Seigo
AU - Kodaira, Ryoji
AU - Ito, Hirataka
PY - 1990/2/14
Y1 - 1990/2/14
N2 - β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.
AB - β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.
UR - http://www.scopus.com/inward/record.url?scp=0025055155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025055155&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91030-V
DO - 10.1016/0006-291X(90)91030-V
M3 - Article
C2 - 2106318
AN - SCOPUS:0025055155
VL - 166
SP - 1453
EP - 1459
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -