Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi; Yasuo Tokushima; Kiyomi Oishi; Yasuyuki Takahashi; Satoshi Shiojiri; Shigenobu Nakamura; Seigo Tanaka; Ryoji Kodaira; Hirataka Ito

doi:10.1016/0006-291X(90)91030-V

Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

Nobuya Kitaguchi
, Yasuo Tokushima
, Kiyomi Oishi
, Yasuyuki Takahashi
, Satoshi Shiojiri
, Shigenobu Nakamura
, Seigo Tanaka
, Ryoji Kodaira
, Hirataka Ito

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

Original language	English
Pages (from-to)	1453-1459
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	166
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)91030-V
Publication status	Published - 14-02-1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(90)91030-V

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Kitaguchi, N., Tokushima, Y., Oishi, K., Takahashi, Y., Shiojiri, S., Nakamura, S., Tanaka, S., Kodaira, R., & Ito, H. (1990). Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. Biochemical and Biophysical Research Communications, 166(3), 1453-1459. https://doi.org/10.1016/0006-291X(90)91030-V

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title = "Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA",

abstract = "β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.",

author = "Nobuya Kitaguchi and Yasuo Tokushima and Kiyomi Oishi and Yasuyuki Takahashi and Satoshi Shiojiri and Shigenobu Nakamura and Seigo Tanaka and Ryoji Kodaira and Hirataka Ito",

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doi = "10.1016/0006-291X(90)91030-V",

language = "English",

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Kitaguchi, N, Tokushima, Y, Oishi, K, Takahashi, Y, Shiojiri, S, Nakamura, S, Tanaka, S, Kodaira, R & Ito, H 1990, 'Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA', Biochemical and Biophysical Research Communications, vol. 166, no. 3, pp. 1453-1459. https://doi.org/10.1016/0006-291X(90)91030-V

Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. / Kitaguchi, Nobuya; Tokushima, Yasuo; Oishi, Kiyomi et al.
In: Biochemical and Biophysical Research Communications, Vol. 166, No. 3, 14.02.1990, p. 1453-1459.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

AU - Kitaguchi, Nobuya

AU - Tokushima, Yasuo

AU - Oishi, Kiyomi

AU - Takahashi, Yasuyuki

AU - Shiojiri, Satoshi

AU - Nakamura, Shigenobu

AU - Tanaka, Seigo

AU - Kodaira, Ryoji

AU - Ito, Hirataka

PY - 1990/2/14

Y1 - 1990/2/14

N2 - β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

AB - β-Amyloid protein precursors (APP) having proteinase inhibitor domains (APPI) were quantified by a new sandwich enzyme linked immunosorbent assay for detection of active (free) form of proteinase inhibitors by using trypsin in place of the first antibody and by denaturation of APPI-trypsin complex in the microtiterplate. The concentration of APPs having APPI in cerebrospinal fluid of Alzheimer's disease patients was found, by this method, to be significantly elevated compared with those of multi-infarct dementia.

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Kitaguchi N, Tokushima Y, Oishi K, Takahashi Y, Shiojiri S, Nakamura S et al. Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA. Biochemical and Biophysical Research Communications. 1990 Feb 14;166(3):1453-1459. doi: 10.1016/0006-291X(90)91030-V

Determination of amyloid β protein precursors harboring active form of proteinase inhibitor domains in cerebrospinal fluid of Alzheimer's disease patients by trypsin-antibody sandwich ELISA

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