Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

T. Nabeshima; M. Hiramatsu; S. Noma; M. Ukai; M. Amano; T. Kameyama

Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

T. Nabeshima, M. Hiramatsu, S. Noma, M. Ukai, M. Amano, T. Kameyama

Research output: Contribution to journal › Article › peer-review

Abstract

High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.

Original language	English
Pages (from-to)	421-442
Number of pages	22
Journal	Research Communications in Chemical Pathology and Pharmacology
Volume	35
Issue number	3
Publication status	Published - 1982
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Pharmacology, Toxicology and Pharmaceutics
Pathology and Forensic Medicine
Toxicology
Pharmacology

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Nabeshima, T., Hiramatsu, M., Noma, S., Ukai, M., Amano, M., & Kameyama, T. (1982). Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector. Research Communications in Chemical Pathology and Pharmacology, 35(3), 421-442.

Nabeshima, T. ; Hiramatsu, M. ; Noma, S. et al. / Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector. In: Research Communications in Chemical Pathology and Pharmacology. 1982 ; Vol. 35, No. 3. pp. 421-442.

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title = "Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector",

abstract = "High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.",

author = "T. Nabeshima and M. Hiramatsu and S. Noma and M. Ukai and M. Amano and T. Kameyama",

year = "1982",

language = "English",

volume = "35",

pages = "421--442",

journal = "Research Communications in Chemical Pathology and Pharmacology",

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}

Nabeshima, T, Hiramatsu, M, Noma, S, Ukai, M, Amano, M & Kameyama, T 1982, 'Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector', Research Communications in Chemical Pathology and Pharmacology, vol. 35, no. 3, pp. 421-442.

Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector. / Nabeshima, T.; Hiramatsu, M.; Noma, S. et al.
In: Research Communications in Chemical Pathology and Pharmacology, Vol. 35, No. 3, 1982, p. 421-442.

Research output: Contribution to journal › Article › peer-review

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T1 - Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

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AU - Amano, M.

AU - Kameyama, T.

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AB - High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.

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Nabeshima T, Hiramatsu M, Noma S, Ukai M, Amano M, Kameyama T. Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector. Research Communications in Chemical Pathology and Pharmacology. 1982;35(3):421-442.

Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

Abstract

All Science Journal Classification (ASJC) codes

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