Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

Toshitaka Nabeshima, M. Hiramatsu, S. Noma, M. Ukai, M. Amano, T. Kameyama

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.

Original languageEnglish
Pages (from-to)421-442
Number of pages22
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume35
Issue number3
Publication statusPublished - 11-06-1982
Externally publishedYes

Fingerprint

High pressure liquid chromatography
3,4-Dihydroxyphenylacetic Acid
Methionine Enkephalin
Homovanillic Acid
Dopamine
Brain
Norepinephrine
High Pressure Liquid Chromatography
Detectors
Rats
Tissue

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

@article{897d3b5ef89c4b5b8913c5e8d934dd40,
title = "Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector",
abstract = "High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.",
author = "Toshitaka Nabeshima and M. Hiramatsu and S. Noma and M. Ukai and M. Amano and T. Kameyama",
year = "1982",
month = "6",
day = "11",
language = "English",
volume = "35",
pages = "421--442",
journal = "Research Communications in Chemical Pathology and Pharmacology",
issn = "0034-5164",
publisher = "PJD Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector

AU - Nabeshima, Toshitaka

AU - Hiramatsu, M.

AU - Noma, S.

AU - Ukai, M.

AU - Amano, M.

AU - Kameyama, T.

PY - 1982/6/11

Y1 - 1982/6/11

N2 - High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.

AB - High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) is same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.

UR - http://www.scopus.com/inward/record.url?scp=0020044383&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020044383&partnerID=8YFLogxK

M3 - Article

VL - 35

SP - 421

EP - 442

JO - Research Communications in Chemical Pathology and Pharmacology

JF - Research Communications in Chemical Pathology and Pharmacology

SN - 0034-5164

IS - 3

ER -