Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song; Dan Ge; Kana Ishii; Hiroshi Yamada; Kazuya Toriumi; Hiroyuki Watanabe; Toshitaka Nabeshima; Takeshi Fukushima

doi:10.1002/bmc.1639

Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song, Dan Ge, Kana Ishii, Hiroshi Yamada, Kazuya Toriumi, Hiroyuki Watanabe, Toshitaka Nabeshima, Takeshi Fukushima

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

Original language	English
Pages (from-to)	147-151
Number of pages	5
Journal	Biomedical Chromatography
Volume	26
Issue number	2
DOIs	https://doi.org/10.1002/bmc.1639
Publication status	Published - 02-2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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title = "Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection",

abstract = "The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.",

author = "Ziyu Song and Dan Ge and Kana Ishii and Hiroshi Yamada and Kazuya Toriumi and Hiroyuki Watanabe and Toshitaka Nabeshima and Takeshi Fukushima",

year = "2012",

month = feb,

doi = "10.1002/bmc.1639",

language = "English",

volume = "26",

pages = "147--151",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "2",

}

Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. / Song, Ziyu; Ge, Dan; Ishii, Kana; Yamada, Hiroshi; Toriumi, Kazuya; Watanabe, Hiroyuki; Nabeshima, Toshitaka; Fukushima, Takeshi.

In: Biomedical Chromatography, Vol. 26, No. 2, 02.2012, p. 147-151.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Song, Ziyu

AU - Ge, Dan

AU - Ishii, Kana

AU - Yamada, Hiroshi

AU - Toriumi, Kazuya

AU - Watanabe, Hiroyuki

AU - Nabeshima, Toshitaka

AU - Fukushima, Takeshi

PY - 2012/2

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Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Abstract

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