Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song, Dan Ge, Kana Ishii, Hiroshi Yamada, Kazuya Toriumi, Hiroyuki Watanabe, Toshitaka Nabeshima, Takeshi Fukushima

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Abstract

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalBiomedical Chromatography
Volume26
Issue number2
DOIs
Publication statusPublished - 01-02-2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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