Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song, Dan Ge, Kana Ishii, Hiroshi Yamada, Kazuya Toriumi, Hiroyuki Watanabe, Toshitaka Nabeshima, Takeshi Fukushima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

Original languageEnglish
Pages (from-to)147-151
Number of pages5
JournalBiomedical Chromatography
Volume26
Issue number2
DOIs
Publication statusPublished - 01-02-2012
Externally publishedYes

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High performance liquid chromatography
Brain
Fluorescence
Olfactory Bulb
High Pressure Liquid Chromatography
Hippocampus
Nucleus Accumbens
Prefrontal Cortex
Cerebellum
N-acetylaspartate
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Song, Z., Ge, D., Ishii, K., Yamada, H., Toriumi, K., Watanabe, H., ... Fukushima, T. (2012). Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. Biomedical Chromatography, 26(2), 147-151. https://doi.org/10.1002/bmc.1639
Song, Ziyu ; Ge, Dan ; Ishii, Kana ; Yamada, Hiroshi ; Toriumi, Kazuya ; Watanabe, Hiroyuki ; Nabeshima, Toshitaka ; Fukushima, Takeshi. / Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. In: Biomedical Chromatography. 2012 ; Vol. 26, No. 2. pp. 147-151.
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Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. / Song, Ziyu; Ge, Dan; Ishii, Kana; Yamada, Hiroshi; Toriumi, Kazuya; Watanabe, Hiroyuki; Nabeshima, Toshitaka; Fukushima, Takeshi.

In: Biomedical Chromatography, Vol. 26, No. 2, 01.02.2012, p. 147-151.

Research output: Contribution to journalArticle

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