Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song; Dan Ge; Kana Ishii; Hiroshi Yamada; Kazuya Toriumi; Hiroyuki Watanabe; Toshitaka Nabeshima; Takeshi Fukushima

doi:10.1002/bmc.1639

Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection

Ziyu Song, Dan Ge, Kana Ishii, Hiroshi Yamada, Kazuya Toriumi, Hiroyuki Watanabe, Toshitaka Nabeshima, Takeshi Fukushima

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8 Citations (Scopus)

Abstract

The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.

Original language	English
Pages (from-to)	147-151
Number of pages	5
Journal	Biomedical Chromatography
Volume	26
Issue number	2
DOIs	https://doi.org/10.1002/bmc.1639
Publication status	Published - 01-02-2012
Externally published	Yes

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All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

Cite this

Song, Z., Ge, D., Ishii, K., Yamada, H., Toriumi, K., Watanabe, H., Nabeshima, T., & Fukushima, T. (2012). Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. Biomedical Chromatography, 26(2), 147-151. https://doi.org/10.1002/bmc.1639

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abstract = "The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.",

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AU - Watanabe, Hiroyuki

AU - Nabeshima, Toshitaka

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10.1002/bmc.1639