Abstract
The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.
| Original language | English |
|---|---|
| Pages (from-to) | 147-151 |
| Number of pages | 5 |
| Journal | Biomedical Chromatography |
| Volume | 26 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 01-02-2012 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Molecular Biology
- Pharmacology
- Drug Discovery
- Clinical Biochemistry
Cite this
}
Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection. / Song, Ziyu; Ge, Dan; Ishii, Kana; Yamada, Hiroshi; Toriumi, Kazuya; Watanabe, Hiroyuki; Nabeshima, Toshitaka; Fukushima, Takeshi.
In: Biomedical Chromatography, Vol. 26, No. 2, 01.02.2012, p. 147-151.Research output: Contribution to journal › Article
TY - JOUR
T1 - Determination of N-acetylaspartic acid concentration in the mouse brain using HPLC with fluorescence detection
AU - Song, Ziyu
AU - Ge, Dan
AU - Ishii, Kana
AU - Yamada, Hiroshi
AU - Toriumi, Kazuya
AU - Watanabe, Hiroyuki
AU - Nabeshima, Toshitaka
AU - Fukushima, Takeshi
PY - 2012/2/1
Y1 - 2012/2/1
N2 - The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.
AB - The concentration of brain N-acetylaspartic acid (NAA) in mice was determined by high-performance liquid chromatography (HPLC) using fluorescence detection after pre-column derivatization with 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benzoxadiazole (DBD-ED). Six different brain parts, namely, the prefrontal cortex, olfactory bulb, nucleus accumbens, striatum, cerebellum and hippocampus, of male C57BL6/J mice, were investigated. The NAA concentration (nmol/mg protein) was highest in the olfactory bulb (58.2±4.0, n=8) and lowest in the hippocampus (42.8±1.6, n=8). The proposed HPLC method with fluorescence detection was successfully used to determine the NAA concentration in each investigated brain area.
UR - http://www.scopus.com/inward/record.url?scp=84855428466&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855428466&partnerID=8YFLogxK
U2 - 10.1002/bmc.1639
DO - 10.1002/bmc.1639
M3 - Article
C2 - 21547934
AN - SCOPUS:84855428466
VL - 26
SP - 147
EP - 151
JO - Biomedical Chromatography
JF - Biomedical Chromatography
SN - 0269-3879
IS - 2
ER -