Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

Yoko S. Kaneko, Keiji Mori, Akira Nakashima, Ikuko Nagatsu, Toshiharu Nagatsu, Akira Ota

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalBrain Research Protocols
Volume8
Issue number1
DOIs
Publication statusPublished - 01-01-2001

Fingerprint

Locus Coeruleus
Biopterin
Fluorescence
High Pressure Liquid Chromatography
sapropterin

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Kaneko, Yoko S. ; Mori, Keiji ; Nakashima, Akira ; Nagatsu, Ikuko ; Nagatsu, Toshiharu ; Ota, Akira. / Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection. In: Brain Research Protocols. 2001 ; Vol. 8, No. 1. pp. 25-31.
@article{2188ffa50b5f4726adc237660045a785,
title = "Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection",
abstract = "Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.",
author = "Kaneko, {Yoko S.} and Keiji Mori and Akira Nakashima and Ikuko Nagatsu and Toshiharu Nagatsu and Akira Ota",
year = "2001",
month = "1",
day = "1",
doi = "10.1016/S1385-299X(01)00081-2",
language = "English",
volume = "8",
pages = "25--31",
journal = "Brain Research Protocols",
issn = "1385-299X",
publisher = "Elsevier BV",
number = "1",

}

Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection. / Kaneko, Yoko S.; Mori, Keiji; Nakashima, Akira; Nagatsu, Ikuko; Nagatsu, Toshiharu; Ota, Akira.

In: Brain Research Protocols, Vol. 8, No. 1, 01.01.2001, p. 25-31.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

AU - Kaneko, Yoko S.

AU - Mori, Keiji

AU - Nakashima, Akira

AU - Nagatsu, Ikuko

AU - Nagatsu, Toshiharu

AU - Ota, Akira

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

AB - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

UR - http://www.scopus.com/inward/record.url?scp=0034887920&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034887920&partnerID=8YFLogxK

U2 - 10.1016/S1385-299X(01)00081-2

DO - 10.1016/S1385-299X(01)00081-2

M3 - Article

VL - 8

SP - 25

EP - 31

JO - Brain Research Protocols

JF - Brain Research Protocols

SN - 1385-299X

IS - 1

ER -