Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

Yoko S. Kaneko, Keiji Mori, Akira Nakashima, Ikuko Nagatsu, Toshiharu Nagatsu, Akira Ota

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalBrain Research Protocols
Volume8
Issue number1
DOIs
Publication statusPublished - 01-01-2001

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All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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