Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

Yoko S. Kaneko; Keiji Mori; Akira Nakashima; Ikuko Nagatsu; Toshiharu Nagatsu; Akira Ota

doi:10.1016/S1385-299X(01)00081-2

Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

Yoko S. Kaneko
, Keiji Mori
, Akira Nakashima
, Ikuko Nagatsu
, Toshiharu Nagatsu
, Akira Ota

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

Original language	English
Pages (from-to)	25-31
Number of pages	7
Journal	Brain Research Protocols
Volume	8
Issue number	1
DOIs	https://doi.org/10.1016/S1385-299X(01)00081-2
Publication status	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience

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10.1016/S1385-299X(01)00081-2

Cite this

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title = "Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection",

abstract = "Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.",

author = "Kaneko, \{Yoko S.\} and Keiji Mori and Akira Nakashima and Ikuko Nagatsu and Toshiharu Nagatsu and Akira Ota",

note = "Funding Information: This work was supported by grants-in-aid from Fujita Health University, Japan, to AO and YSK. We thank Ms. Hiromi Hasegawa, Chisako Nakagawa, Mayumi Hirashita, and Naomi Yazawa for their technical help.",

year = "2001",

doi = "10.1016/S1385-299X(01)00081-2",

language = "English",

volume = "8",

pages = "25--31",

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TY - JOUR

T1 - Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

AU - Kaneko, Yoko S.

AU - Mori, Keiji

AU - Nakashima, Akira

AU - Nagatsu, Ikuko

AU - Nagatsu, Toshiharu

AU - Ota, Akira

N1 - Funding Information: This work was supported by grants-in-aid from Fujita Health University, Japan, to AO and YSK. We thank Ms. Hiromi Hasegawa, Chisako Nakagawa, Mayumi Hirashita, and Naomi Yazawa for their technical help.

PY - 2001

Y1 - 2001

N2 - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

AB - Tetrahydrobiopterin in the murine locus coeruleus was measured as its fully oxidized form, biopterin, using a HPLC coupled to a fluorescence detector, because tetrahydrobiopterin itself cannot be detected by such means. The differential oxidization method distinguished tetrahydrobiopterin-derived biopterin and dihydrobiopterin-derived biopterin. The protocol reported here is a rapid and sensitive method that facilitates the measurement of tissue and/or cellular tetrahydrobiopterin. Using this assay protocol, we were able to detect and quantify variations in the tetrahydrobiopterin content in the murine locus coeruleus.

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JO - Brain Research Protocols

JF - Brain Research Protocols

IS - 1

ER -

Determination of tetrahydrobiopterin in murine locus coeruleus by HPLC with fluorescence detection

Abstract

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