Determining the nucleotide sequence and capsid-coding region of Himetobi P virus

A member of a novel group of RNA viruses that infect insects

N. Nakashima, Jun Sasaki, S. Toriyama

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We determined the complete genome sequence of Himetobi P virus (HiPV), an insect picorna-like virus, which was isolated from the small brown planthopper, Laodelphax striatellus. The genome of HiPV consists of 9,275 nucleotides excluding the poly (A) tail, and contains two large open reading frames (ORFs), which were separated by a 176-nucleotide noncoding region. The deduced amino acid sequence of the first ORF contains core motifs of picornaviral helicase, protease, and RNA-dependent RNA polymerase. The capsid protein-coding region was mapped onto the second ORF by determining the N-terminal amino acid sequences of the capsid proteins. Subgenomic RNA for the capsid protein gene was not detected in the infected tissue. The capsid protein precursor gene of HiPV lacks an AUG initiation codon at the expected position and the upstream sequence of the gene is predicted to form several stem-loop structures, suggesting that the precursor is produced by internal ribosome entry site (IRES) mediated-translation, as occurs in Plutia stali intestine virus (PSIV). These characteristics of the HiPV genome are similar to those of a new group of RNA viruses consisting of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), and PSIV.

Original languageEnglish
Pages (from-to)2051-2058
Number of pages8
JournalArchives of Virology
Volume144
Issue number10
DOIs
Publication statusPublished - 08-12-1999

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Capsid
RNA Viruses
Insects
Viruses
Capsid Proteins
Open Reading Frames
Genome
Intestines
Amino Acid Sequence
Nucleotides
Insect Viruses
Genes
RNA Replicase
Protein Precursors
Initiator Codon
Drosophila
Peptide Hydrolases
RNA
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

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abstract = "We determined the complete genome sequence of Himetobi P virus (HiPV), an insect picorna-like virus, which was isolated from the small brown planthopper, Laodelphax striatellus. The genome of HiPV consists of 9,275 nucleotides excluding the poly (A) tail, and contains two large open reading frames (ORFs), which were separated by a 176-nucleotide noncoding region. The deduced amino acid sequence of the first ORF contains core motifs of picornaviral helicase, protease, and RNA-dependent RNA polymerase. The capsid protein-coding region was mapped onto the second ORF by determining the N-terminal amino acid sequences of the capsid proteins. Subgenomic RNA for the capsid protein gene was not detected in the infected tissue. The capsid protein precursor gene of HiPV lacks an AUG initiation codon at the expected position and the upstream sequence of the gene is predicted to form several stem-loop structures, suggesting that the precursor is produced by internal ribosome entry site (IRES) mediated-translation, as occurs in Plutia stali intestine virus (PSIV). These characteristics of the HiPV genome are similar to those of a new group of RNA viruses consisting of Drosophila C virus (DCV), Rhopalosiphum padi virus (RhPV), and PSIV.",
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Determining the nucleotide sequence and capsid-coding region of Himetobi P virus : A member of a novel group of RNA viruses that infect insects. / Nakashima, N.; Sasaki, Jun; Toriyama, S.

In: Archives of Virology, Vol. 144, No. 10, 08.12.1999, p. 2051-2058.

Research output: Contribution to journalArticle

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